SRP4696

Sigma-Aldrich

ApoE3 human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE), ≥90% (HPLC)

Sinónimos:
Apolipoprotein E3
Número de CAS:
MDL number:
NACRES:
NA.32
En este momento no podemos mostrarle ni los precios ni la disponibilidad

biological source

human

recombinant

expressed in E. coli

assay

≥90% (HPLC)
≥90% (SDS-PAGE)

form

lyophilized

mol wt

~34 kDa

packaging

pkg of 500 μg

storage condition

avoid repeated freeze/thaw cycles

application(s)

activity assay: suitable

impurities

endotoxin, tested

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... APOE(348)

General description

Significant quantities of ApoE are produced in liver and brain and to some extent in almost every organ. ApoE is an important constituent of all plasma lipoproteins. It′s interaction with specific ApoE receptor enables uptake of chylomicron remnants by liver cells, which is an essential step during normal lipid metabolism. ApoE exists in three major isoforms; E2, E3, and E4, which differ from one another by a single amino-acid substitution. E3 is the most common isoform and is present in 40-90% of the population. Recombinant human ApoE3 is a 34.0 kDa protein containing 299 amino acid residues. The recombinant ApoE3 was manufactured using animal origin free technology.

Application

Human ApoE has been used to study the role of apoE in the activity of LNP (lipid nanoparticles) in order to determine if ApoE has a role to play in the delivery of siRNA to hepatocytes.

Biochem/physiol Actions

ApoE belongs to a group of proteins that bind reversibly with lipoprotein and play an important role in lipid metabolism. In addition to facilitating solublization of lipids, these proteins help to maintain the structural integrity of lipoproteins, serve as ligands for lipoprotein receptors, and regulate the activity of enzymes involved in lipid metabolism. Apart from participating in the metabolism of plasma lipoproteins, apo-E also interacts with several proteoglycans, such as heparin. The interaction of lipoproteins with proteoglycans in the walls of arteries has been associated with cholesterol deposition linked to atherosclerosis. It is found to be a constituent of several lipoproteins and serves as a ligand for the LDL (low density lipoprotein) receptor, thereby regulating cholesterol and triglyceride homeostasis.

Physical form

Sterile filtered and lyophilized from 20 mM sodium phosphate.

Reconstitution

Reconstitute in 5 mM Sodium Phosphate pH 7.8 + 0.5 mM DTT to a concentration of 0.1-1.0 mg/mL. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificado de Análisis
Human apolipoprotein E. Determination of the heparin binding sites of apolipoprotein E3.
Weisgraber KH
The Journal of Biological Chemistry, 261, 2068-2076 (1986)
Yuta Suzuki et al.
International journal of pharmaceutics, 510(1), 350-358 (2016-07-05)
Lipid nanoparticles (LNPs) represent the most advanced platform for the systemic delivery of siRNA. We have previously reported the discovery of novel ionizable lipids with asymmetric lipid tails, enabling potent gene-silencing activity in hepatocytes in vivo; however, the structure and...
Emmanuel U Okoro et al.
Biochemical and biophysical research communications, 477(1), 123-128 (2016-06-15)
We previously reported that apolipoprotein E (apoE) upregulates ATP-binding cassette transporter A1 (ABCA1) transcription through phosphatidylinositol 3-kinase (PI3K). Here we demonstrate that treatment of murine macrophages with human apoE3 enhanced Akt phosphorylation, and upregulated ABCA1 protein and mRNA expression. Inhibition...

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico

Redes sociales

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

Merck

Investigación. Desarrollo. Producción.

Somos un proveedor líder para la industria de Ciencias de la Vida con soluciones y servicios para investigación, desarrollo y producción biotecnológicos, y para desarrollo y producción de tratamientos farmacéuticos

© 2021 Merck KGaA, Darmstadt, Alemania y/o sus filiales. Todos los derechos reservados.

Queda estrictamente prohibida la reproducción sin permiso de cualquiera de los materiales de la página web.