SRP5085

Sigma-Aldrich

SIK (1-303), active, His tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Sinónimos:
SNF1LK, MSK, SIK1
NACRES:
NA.32
En este momento no podemos mostrarle ni los precios ni la disponibilidad

recombinant

expressed in baculovirus infected Sf9 cells

product line

PRECISIO® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

296-401 nmol/min·mg

mol wt

~36 kDa

NCBI accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... SIK1(150094)

General description

SIK is a protein kinase that is involved in regulating AMPK-related kinases. SIK may mediate the physiological effects of LKB1, including its tumour suppressor function. SIK is also involved in signaling by various proteins like STRAD, NUAK1, NUAK2, BRSK1, BRSK2, QIK, QSK, SIK, MARK1, MARK2, MARK3, MARK4 and MELK that are related to AMPK. Activation of SIK1 by phosphorylation on thr322 can lead to an increase in the catalytic activity of sodium/potassium ATPase alpha subunit at the plasma membrane. This results in an increase in intracellular sodium in intact mammalian cells.

Physical form

Supplied in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Legal Information

PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC

pictograms

Exclamation markHealth hazard

signalword

Danger

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point F

Not applicable

Flash Point C

Not applicable

Hiroshi Takemori et al.
The FEBS journal, 274(13), 3202-3209 (2007-06-15)
The transcription factor cAMP response element-binding protein (CREB) plays important roles in gene expression induced by cAMP signaling and is believed to be activated when its Ser133 is phosphorylated. However, the discovery of Ser133-independent activation by the activation of transducer...
Mattias Sjöström et al.
Proceedings of the National Academy of Sciences of the United States of America, 104(43), 16922-16927 (2007-10-18)
In mammalian cells, active sodium transport and its derived functions (e.g., plasma membrane potential) are dictated by the activity of the Na(+),K(+)-ATPase (NK), whose regulation is essential for maintaining cell volume and composition, as well as other vital cell functions....

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