Merck
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SRP5132

Sigma-Aldrich

SMAD3, GST tagged human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):
DKFZp686J10186, JV15-2, HsT17436, MADH3, HSPC193, MGC60396, DKFZP586N0721
CAS Number:
NACRES:
NA.32

origen biológico

human

recombinante

expressed in E. coli

ensayo

≥70% (SDS-PAGE)

formulario

buffered aqueous glycerol solution

mol peso

~77 kDa

Nº de acceso NCBI

application(s)

cell analysis

enviado en

dry ice

temp. de almacenamiento

−70°C

Gene Information

human ... SMAD3(4088)

Descripción general

SMAD3 is a direct mediator of transcriptional activation by the TGFβ receptor. The activity of SMAD3 is regulated by the TGFβ receptors, and SMAD3 is phosphorylated and associated with the ligand-bound receptor complex. TGFβ stimulation leads to phosphorylation and activation of SMAD3, which form a complex with SMAD4 that accumulate in the nucleus and regulate transcription of target genes such as CDK inhibitor. SMAD3 containing a C-terminal truncation acts as a dominant-negative inhibitor of the normal TGFβ response. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2.

Forma física

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Nota de preparación

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Código de clase de almacenamiento

10 - Combustible liquids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

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Certificate of Origin

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