Merck
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SRP6103

Sigma-Aldrich

MDH1 human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE)

Synonym(s):
MDH-s, MDHA, MOR2, Malate dehydrogenase cytoplasmic
CAS Number:
NACRES:
NA.32

origen biológico

human

recombinante

expressed in E. coli

ensayo

≥95% (SDS-PAGE)

formulario

liquid

mol peso

37.4 kDa (342 aa, 1-334 aa + CT His Tag)

envase

pkg of 100 μg

technique(s)

activity assay: suitable

Nº de acceso NCBI

enviado en

dry ice

temp. de almacenamiento

−70°C

Gene Information

human ... MDH1(4190)

Descripción general

MDH1 (malate dehydrogenase 1) is cytoplasmic protein belonging to the 2-hydroxy acid dehydrogenases protein family. Eukaryotes contain two forms of MDH proteins depending upon their subcellular localization, MDH1 and MDH2. MDH2 is present in the mitochondrial matrix and MDH1 is present in the cytoplasm. This gene is localized to human chromosome 2, and is composed of nine exons and eight introns.

Aplicación

MDH1 (malate dehydrogenase 1) human has been used for measuring MDH1 enzyme activity.

Acciones bioquímicas o fisiológicas

MDH1 (malate dehydrogenase 1) is responsible for reversibly converting malate to oxaloacetate in cytoplasm. It participates in metabolism where it transports NADH equivalents and metabolites across the mitochondrial membrane, and regulates the TCA (tricarboxylic acid) cycle pool size. MDH1 is involved in malate/aspartate shuttle, which is involved in maintaining the balance of nitrogen, oxaloacetate, and the α-ketoglutarate intermediate shuttling between the cytosol and mitochondrial matrix. It is thought to function as an important checkpoint for energy balance between mitochondria and cytoplasm. MDH1 acts as a regulator of p53-dependent apoptosis in response to glucose deprivation, indicating that synergism between energy metabolism and p53 transactivation is linked with cell metabolic state and hence, determination of cell death.

Forma física

1 mg/mL solution in 20 mM Tris-HCl (pH 8.0) containing 10% glycerol.

Nota de preparación

Centrifuge the vial prior to opening.

Otras notas

MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL DGVLMELQDC ALPLLKDVIA TDKEDVAFKD LDVAILVGSM PRREGMERKD LLKANVKIFK SQGAALDKYA KKSVKVIVVG NPANTNCLTA SKSAPSIPKE NFSCLTRLDH NRAKAQIALK LGVTANDVKN VIIWGNHSST QYPDVNHAKV KLQGKEVGVY EALKDDSWLK GEFVTTVQQR GAAVIKARKL SSAMSAAKAI CDHVRDIWFG TPEGEFVSMG VISDGNSYGV PDDLLYSFPV VIKNKTWKFV EGLPINDFSR EKMDLTAKEL TEEKESAFEF LSSALEHHHH HH

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

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Certificate of Origin

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