SRP6253

Sigma-Aldrich

EGF human

recombinant, expressed in HEK 293 cells, ≥97% (SDS-PAGE)

Sinónimos:
URG, epidermal growth factor, EGF, HOMG4
NACRES:
NA.32
En este momento no podemos mostrarle ni los precios ni la disponibilidad

biological source

human

recombinant

expressed in HEK 293 cells

assay

≥97% (SDS-PAGE)

form

lyophilized powder

mol wt

calculated mol wt 6.3 kDa
observed mol wt 6.3 kDa by SDS-PAGE (DTT-reduced.)

packaging

pkg of 10 μg

impurities

<1 EU/μg endotoxin (LAL test)

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... EGF(1950)

Categorías relacionadas

General description

The gene EGF (epidermal growth factor) is mapped to human chromosome 4q25-27. The gene encodes for a large type I membrane–anchored precursor protein which is cleaved into pro-EGF for generation of peptide hormone EGF. The mRNA is expressed in the kidney, salivary gland, cerebrum and prostate. Recombinant human EGF is a 6.2kDa globular protein containing 53 amino acid residues including 3 intramolecular disulfide-bonds.

Application

EGF human has been used to check the action of EGF on lung epithelial NOS (nitric oxide synthase) in A549 cells (adenocarcinoma human alveolar basal epithelial cells). It has been used in the tumor sphere medium for culturing of brain tumor cells.

Biochem/physiol Actions

EGF (epidermal growth factor) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. It signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF (transforming growth factor)-α and VGF (vaccinia virus growth factor). Mutation in the EGF gene might be associated with risk of non-small cell lung adenocarcinoma. It is also involved in anaplastic thyroid cancer and renal hypomagnesemia. EGF controls cell differentiation and neurotrophic processes.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificado de Análisis
Constitutive and inducible nitric oxide synthase gene expression, regulation, and activity in human lung epithelial cells.
Asano K, et al.
Proceedings of the National Academy of Sciences of the USA, 91, 10089-10093 (1994)
Identification of a cancer stem cell in human brain tumors.
Singh SK, et al.
Cancer Research, 63, 5821-5828 (2003)
Clinical Implication of EGF A61G Polymorphism in the Risk of Non Small Cell Lung Adenocarcinoma Patients: A Case Control Study.
Masroor M
Asian Pacific Journal of Cancer Prevention, 16, 7529-7534 (2015)
The miR-200 family regulates the epithelial-mesenchymal transition induced by EGF/EGFR in anaplastic thyroid cancer cells.
Zhang Z
International Journal of Molecular Medicine, 30, 856-862 (2012)
Neurotrophic actions of a novel molluscan epidermal growth factor.
Hermann PM
The Journal of Neuroscience, 20, 6355-6364 (2000)

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