Merck
All Photos(1)

SRP6415

Sigma-Aldrich

Cathepsin D human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Synonym(s):
CLN10, CPSD, CTSD
NACRES:
NA.32

origen biológico

human

recombinante

expressed in HEK 293 cells

etiqueta

6-His tagged (C-terminus)

ensayo

≥95% (SDS-PAGE)

formulario

lyophilized

mol peso

calculated mol wt 43.6 kDa
observed mol wt 45-55 kDa (DTT-reduced. Protein migrates due to glycosylation. Ser 19 is the predicted N-terminal.)

envase

pkg of 10 μg

impurezas

<1 EU/μg endotoxin (LAL test)

Nº de acceso UniProt

enviado en

wet ice

temp. de almacenamiento

−20°C

Gene Information

human ... CTSD(1509)

Descripción general

Cathepsin D is also known as CTSD, CPSD, which belongs to the peptidase A1 family. Cathepsin D can be cleaved into the following 2 chains: cathepsin D light chain and cathepsin D heavy chain, which is expressed in the aorta extracellular space (at protein level). The catalytic activity of Cathepsin D is specificity similar to, but narrower than, that of pepsin A. Cathepsin D does not cleave the 4-Gln-|-His-5 bond in B chain of insulin. Cathepsin D involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
This gene is mapped to human chromosome 11p15.5.[1]

Acciones bioquímicas o fisiológicas

Cathepsin D (CatD) takes part in the intracellular degradation of advanced glycation end (AGE) products. AGE deposition is predominant in photoaged skin. Thus, CatD might be useful in antiphotoaging therapy.[2] Elevated circulating CatD is observed in type 2 diabetes and can be considered as an important biomarker for type 2 diabetes dependent cardiac dysfunction.[3] Increased CatD, is observed in non-alcoholic steatohepatitis.[4]

Forma física

Lyophilized from 0.22 μm filtered solution in 50 mM MES, pH 6.5 with 100 mM NaCl. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitución

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service