SRP6415

Cathepsin D human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonym(s): CLN10, CPSD, CTSD
• NACRES: NA.32

Properties

• origen biológico: human
• recombinante: expressed in HEK 293 cells
• etiqueta: 6-His tagged (C-terminus)
• ensayo: ≥95% (SDS-PAGE)
• formulario: lyophilized
• mol peso: calculated mol wt 43.6 kDa; observed mol wt 45-55 kDa (DTT-reduced. Protein migrates due to glycosylation. Ser 19 is the predicted N-terminal.)
• envase: pkg of 10 μg
• impurezas: <1 EU/μg endotoxin (LAL test)
• Nº de acceso UniProt: P07339
• enviado en: wet ice
• temp. de almacenamiento: −20°C
• Gene Information: human ... CTSD(1509)

Description

Descripción general

Cathepsin D is also known as CTSD, CPSD, which belongs to the peptidase A1 family. Cathepsin D can be cleaved into the following 2 chains: cathepsin D light chain and cathepsin D heavy chain, which is expressed in the aorta extracellular space (at protein level). The catalytic activity of Cathepsin D is specificity similar to, but narrower than, that of pepsin A. Cathepsin D does not cleave the 4-Gln-|-His-5 bond in B chain of insulin. Cathepsin D involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.

This gene is mapped to human chromosome 11p15.5.[1]

Acciones bioquímicas o fisiológicas

Cathepsin D (CatD) takes part in the intracellular degradation of advanced glycation end (AGE) products. AGE deposition is predominant in photoaged skin. Thus, CatD might be useful in antiphotoaging therapy.[2] Elevated circulating CatD is observed in type 2 diabetes and can be considered as an important biomarker for type 2 diabetes dependent cardiac dysfunction.[3] Increased CatD, is observed in non-alcoholic steatohepatitis.[4]

Forma física

Lyophilized from 0.22 μm filtered solution in 50 mM MES, pH 6.5 with 100 mM NaCl. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitución

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety Information

• Código de clase de almacenamiento: 11 - Combustible Solids
• WGK: WGK 3
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable