Merck
All Photos(2)

SRP6448

Sigma-Aldrich

Fas Ligand/FasL human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Synonym(s):
ALPS1B, APT1LG1, CD178, CD95-L, CD95L, FASL, FASLG, Fas ligand, TNFSF6
NACRES:
NA.32

origen biológico

human

recombinante

expressed in HEK 293 cells

etiqueta

6-His tagged (N-terminus)

ensayo

≥95% (SDS-PAGE)

formulario

lyophilized

mol peso

calculated mol wt 17.7 kDa
observed mol wt 25-32 kDa by SDS-PAGE (reducing) (His is the predicted N-terminal .)

envase

pkg of 10 μg

impurezas

<1 EU/μg endotoxin (LAL test)

Nº de acceso UniProt

enviado en

wet ice

temp. de almacenamiento

−20°C

Gene Information

human ... FASLG(356)

Descripción general

Fas ligand also known as FasL, CD178, CD95L, or TNFSF6, is a 40 kDa homotrimeric type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. The FASL gene was mapped to human chromosome 1q24.3. It spans a length of 8 kb and contains 4 exons. It is found to be expressed on T and NK cells that are activated.

Acciones bioquímicas o fisiológicas

The binding of Fas ligand to its receptor induces apoptosis. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Its functions include T-cell homeostasis, cytotoxic T-cell activity, immune privilege, maternal tolerance, tumor counterattack. Defective Fas-mediated apoptosis may lead to oncogenesis as well as drug resistance in existing tumors. Germline mutation of Fas is associated with autoimmune lympho proliferative syndrome (ALPS), a childhood disorder of apoptosis.

Forma física

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH 7.0. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitución

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

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Certificate of Origin

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