SRP6505

Sigma-Aldrich

G6PD human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE)

Sinónimos:
Glucose-6-phosphate 1-dehydrogenase, Zwf
NACRES:
NA.32
En este momento no podemos mostrarle ni los precios ni la disponibilidad

biological source

human

recombinant

expressed in E. coli

assay

≥95% (SDS-PAGE)

form

liquid

mol wt

61.4 kDa (515 aa, 1- 515 aa)

packaging

pkg of 100 μg

concentration

0.5 mg/mL

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... G6PD(2539)

General description

G6PD (glucose-6-phosphate dehydrogenase) gene is mapped to human chromosome Xq28, and spans 16.2kb. One G6PD monomer is composed of 515 amino acids with a predicted molecular weight of 59,256Da. The active enzyme exists as a dimer, and contains an NADP molecule tightly bound to it.

Biochem/physiol Actions

G6PD (glucose-6-phosphate dehydrogenase) enzyme catalyzes the oxidation of glucose-6-phosphate to 6-phosphogluconolactone, reducing NADP to NADPH. It therefore, catalyzes the first step of hexose monophosphate pathway (HMP). As it is responsible for the oxidation of glucose-6-phosphate, and thus, confers protection against oxidative damage in erythrocytes. Its deficiency is highly heterogenous, with around 190 variants being reported. The deficiency of G6PD was discovered while studying hemolytic anemia. G6PD also results in hereditary nonspherocytic hemolytic anemia.

Physical form

0.5 mg/mL solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1 mM PMSF, 2 mM EDTA, 2 mM DTT, 200 mM NaCl.

Analysis Note

The biological activity is > 7 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 μmole D-glucose-6-phosphate to 6-phospho-Dgluconate per min in the presence of beta-NADP at pH 7.4 at 25 °C.

pictograms

Exclamation mark

signalword

Warning

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificado de Análisis
Glucose-6-phosphate dehydrogenase deficiency: a historical perspective.
Beutler E
Blood, 111(1), 16-24 (2008)
African glucose-6-phosphate dehydrogenase alleles associated with protection from severe malaria in heterozygous females in Tanzania.
Manjurano A, et al.
PLoS Genetics, 11(2) (2015)
G6PD deficiency.
E Beutler
Blood, 84(11), 3613-3636 (1994-12-01)
Saúl Gómez-Manzo et al.
International journal of molecular sciences, 15(11), 21179-21201 (2014-11-20)
Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common enzyme deficiency worldwide, causing a wide spectrum of conditions with severity classified from the mildest (Class IV) to the most severe (Class I). To correlate mutation sites in the G6PD with the...
E Y Chen et al.
Human molecular genetics, 5(5), 659-668 (1996-05-01)
DNA comprising 219 447 bp was sequenced in nine cosmids and verified at > 99.9% precision. Of the standard repetitive elements, 187 Alus make up 20.6% of the sequence, but there were only 27 MERs (2.9%) and 17 L1 fragments...

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