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Sigma-Aldrich

Enhanced Avian First Strand Synthesis Kit

Components for cDNA synthesis with enhanced AMV reverse transcriptase

NACRES:
NA.55

Quality Level

usage

sufficient for 50 reactions
1 kit sufficient for 50 reactions

feature

dNTPs included
hotstart: no

application(s)

RT-PCR: suitable

color

colorless

input

purified RNA

shipped in

dry ice

storage temp.

−20°C

Application

Enhanced Avian First Strand Synthesis Kit utilizes a highly purified avian myeloblastosis virus reverse transcriptase (eAMV-RT) that offers superior performance in comparison to standard AMV-RT or standard Moloney murine leukemia virus reverse transcriptase (MMLV-RT). This exceptionally robust eAMV-RT has an enhanced ability to transcribe through difficult secondary structure at elevated temperatures (up to 65 °C) making it the ideal enzyme for producing high quality full-length cDNA from total RNA or poly(A)+ RNA.

Unit Definition

One unit incorporates one nanomole of TMP into TCA-precipitable material in 10 minutes using polyadenylic acid as template and oligo(dT)12-18 as primer.

Legal Information

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

Solo componentes del kit

Referencia del producto
Descripción

  • Enhanced Avian Reverse Transcriptase 1000 U

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS

  • Deoxynucleotide mix 50 μL

  • O4387Anchored oligo (dT)23 100 μL

  • R7647Random nonamers 100 μL

  • O4387Ribonuclease inhibitor 50 μL

  • W1754PCR grade water 1.5 mL

Storage Class Code

10 - Combustible liquids

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificado de Análisis

Certificado de origen

Sigma-Aldrich Corporation's Life Science Quarterly. Hot Start RT-PCR results in improved performance of the enhanced Avian RT-PCR
Easlund, E., and Mueller, E.
Sigma data, 2-5 (2001)
María José Martín et al.
The Journal of biological chemistry, 282(47), 34510-34524 (2007-09-21)
Heme oxygenase-1 (HO-1), an inducible enzyme that metabolizes the heme group, is highly expressed in human Kaposi sarcoma lesions. Its expression is up-regulated by the G protein-coupled receptor from the Kaposi sarcoma-associated herpes virus (vGPCR). Although recent evidence shows that...
Julio Carrion et al.
Journal of immunology (Baltimore, Md. : 1950), 189(6), 3178-3187 (2012-08-15)
The low-grade oral infection chronic periodontitis (CP) has been implicated in coronary artery disease risk, but the mechanisms are unclear. In this study, a pathophysiological role for blood dendritic cells (DCs) in systemic dissemination of oral mucosal pathogens to atherosclerotic...
Aerosol-administered α-tocopherol attenuates lung inflammation in rats given lipopolysaccharide intratracheally.
Experimental Lung Research, 31, 34510-34524 (2007)
E M Brooks et al.
BioTechniques, 19(5), 806-812 (1995-11-01)
The secondary structure in mRNA is essential for many processes, but it can present a technical problem in making full-length cDNA with reverse transcriptases. Furthermore, different reverse transcriptases have differing abilities to transcribe through regions with secondary structure, which can...

Artículos

Reverse Transcription

One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in half life between different transcripts, the temporal patterns of transcription and the lack of correlation between mRNA and protein.

Protocolos

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay is a potential first step in the identification of RNA degradation. The assay is particularly useful when a large number of samples are to be analyzed or when the degradation is less than that detected by capillary systems but still sufficient to effect qPCR analyses.

Contenido relacionado

PCR Applications

Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.

RT-qPCR – Quantitative Reverse Transcription PCR

RT-qPCR, or quantitative reverse transcription PCR, combines the effects of reverse transcription and quantitative PCR or real-time PCR to amplify and detect specific targets. RT-qPCR has a variety of applications including quantifying gene expression levels, validating RNA interference (RNAi), and detecting pathogens such as viruses.

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