rat, mouse, human
flow cytometry: suitable
western blot: suitable
human ... TGFB1(7040)
mouse ... Tgfb1(21803)
rat ... Tgfb1(59086)
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Yes, this antibody will neutralize TGF-ß1.
This antibody can be used with both formalin-fixed paraffin-embedded (FFPE) tissue sections and frozen sections.
This antibody can be used for intracellular flow cytometry. The cells should be fixed with 4% paraformaldehyde and permeabilized with a 0.1% saponin-containing buffer.
These two antibodies are very similar in regards to the applications they are suitable for. They do differ in a few key areas. T0688 only recognizes active TGF-ß1 while T0438 recognizes both active and latent TGF-ß1. Also, T0438 is a mouse monoclonal antibody and T0688 is a chicken polyclonal antibody.
TGF-ß1 is a disulfide-linked homodimer of two 12.5 kDa subunits. Under non-reducing conditions, the T0438 antibody should detect a protein at 25 kDa.
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