T0438

Sigma-Aldrich

Monoclonal Anti-Transforming Growth Factor-β1 antibody produced in mouse

clone 9016, purified immunoglobulin, lyophilized powder

Sinónimos:
Anti-TGF-β1, CEDLAP, latency-associated peptide, DPD1
MDL number:
NACRES:
NA.41
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9016, monoclonal

form

lyophilized powder

species reactivity

rat, mouse, human

application(s)

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
neutralization: suitable
western blot: suitable

isotype

IgG1

conjugate

unconjugated

Featured Industry

Research Pathology

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... TGFB1(7040)
mouse ... Tgfb1(21803)
rat ... Tgfb1(59086)

General description

Transforming Growth Factor-β1 (TGFβ1) is mapped to human chromosome 19q13.2.
Transforming growth factor-β (TGF-β) belongs to the TGF-β superfamily of secreted proteins. TGF-β 1 exists as three isoforms TGF-β 1, TGF-β 2 and TGF-β 3. The TGF-β family is involved in a variety of signaling pathways that involve serine/threonine protein kinases. TGF-β acts through binding to three transmembrane receptors in mammals, type I, type II and type III receptors. The important pathways regulated by TGF-β are the SMAD, BMP, MAPK and PI3K/Akt pathways. TGF-β 1 is involved in cell and tissue differentiation, proliferation, inflammation, migration of monocytes, neutrophils and chemotaxis. Deregulation of TGF-β signaling has been implicated in many inflammation and autoimmune diseases. Monoclonal Anti-Transforming Growth Factor-β 1 antibody specifically reacts with human, mouse and rat TGF-β 1. The antibody shows less than 2% cross reactivity with TGF-β3 and TGF-β5 in ELISA.

Specificity

By capture ELISA, <2% cross-reactivity is observed with TGF-β3 and TGF-β5.

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant TGF- beta 1 and latent TGF- beta 1

Application

Monoclonal Anti-Transforming Growth Factor-β1 antibody produced in mouse has been used in:
  • immunohistochemical detection of TGFβ1 in smooth muscle(2) and liver sections
  • western blotting and ELISA of brain specimens
  • immunoblot detection of TGFβ1 in macrophages

Recommended concentrations:
Western Blot 1 ug/mL (under non-reducing conditions only)
Immunohistochemistry 3-25 μg/mL
Flow Cytometry 2.5 μg/106 cells
ELISA capture 2-8 μg/mL
ELISA detection 0.1-0.4 μg/mL
Neutrilization 0.3-1.0 μg/mL

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Other Notes

Protein A or G purified from hybridoma culture supernatant.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point F

Not applicable

Flash Point C

Not applicable

  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. Can Product T0438, Monoclonal Anti-Transforming Growth Factor-?1 antibody produced in mouse, be used to neutralize the biological activity of TGF-ß1?

    Yes, this antibody will neutralize TGF-ß1.  

  7. Can Product T0438, Monoclonal Anti-Transforming Growth Factor-?1 antibody produced in mouse, be used for immunohistochemistry with frozen and/or formalin-fixed paraffin-embedded sections?

    This antibody can be used with both formalin-fixed paraffin-embedded (FFPE) tissue sections and frozen sections.

  8. Will Product T0438, Monoclonal Anti-Transforming Growth Factor-?1 antibody produced in mouse, work for intracellular flow cytometry?

    This antibody can be used for intracellular flow cytometry. The cells should be fixed with 4% paraformaldehyde and permeabilized with a 0.1% saponin-containing buffer.

  9. How is Product T0438, Monoclonal Anti-Transforming Growth Factor-?1 antibody produced in mouse, different than T0688,  Anti-Transforming Growth Factor-?1 antibody produced in chicken?

    These two antibodies are very similar in regards to the applications they are suitable for. They do differ in a few key areas. T0688 only recognizes active TGF-ß1 while T0438 recognizes both active and latent TGF-ß1. Also, T0438 is a mouse monoclonal antibody and T0688 is a chicken polyclonal antibody.

  10. What is the molecular weight of Product T0438, Monoclonal Anti-Transforming Growth Factor-?1 antibody produced in mouse,?

    TGF-ß1 is a disulfide-linked homodimer of two 12.5 kDa subunits. Under non-reducing conditions, the T0438 antibody should detect a protein at 25 kDa.

Alba Di Pardo et al.
Molecular brain, 6, 55-55 (2013-12-18)
Huntington Disease (HD) is a neurodegenerative disorder resulting from the expansion of polyglutamine stretch in the huntingtin protein (Htt). Mutant HTT (mHtt) leads to progressive impairment of several molecular pathways that have been linked to disease pathogenesis. Defects in the...
Carla L Busceti et al.
Frontiers in pharmacology, 8, 111-111 (2017-03-30)
Dkk-3 is a member of the dickkopf protein family of secreted inhibitors of the Wnt pathway, which has been shown to enhance angiogenesis. The mechanism underlying this effect is currently unknown. Here, we used cultured HUVECs to study the involvement...
The potential antifibrotic impact of apocynin and alpha-lipoic acid in concanavalin A-induced liver fibrosis in rats: Role of NADPH oxidases 1 and 4.
Fayed MR, et al.
Drug Discoveries & Therapeutics, 12(2), 58-67 (2018)
Mostafa R Fayed et al.
Drug discoveries & therapeutics, 12(2), 58-67 (2018-05-16)
Liver fibrosis results from chronic inflammation that precipitates excessive accumulation of extracellular matrix. Oxidative stress is involved in its pathogenesis. This study aimed to elucidate the potential antifibrotic effect of the NADPH oxidase (NOX) inhibitor, apocynin against concanavalin A (ConA)-induced...
The transforming growth factor-beta1 (TGFB1) gene is associated with chronic obstructive pulmonary disease (COPD).
Celedon JC, et al.
Human Molecular Genetics, 13(15), 1649-1656 (2004)

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