This product is a ready-to-use peroxidase substrate containing TMB in a mildly acidic buffer. The concentration of the TMB and the formulation of the buffer are considered proprietary.
T0440
3,3′,5,5′-Tetramethylbenzidine
peroxidase substrate, chromogenic, liquid
Sinónimos:
TMB
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100 ML
MXP 4,326.00
1 L
MXP 20,872.00
5 X 1 L
MXP 79,162.00
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UNSPSC Code:
12352204
NACRES:
NA.83
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Permítanos ayudarleNombre del producto
Sistema de sustrato líquido 3,3′,5,5′-tetrametilbencidina (TMB) para ELISA, peroxidase substrate
form
liquid
storage temp.
2-8°C
Quality Level
Categorías relacionadas
1 of 4
Este artículo | T0565 | T5569 | T4444 |
|---|---|---|---|
| Quality Level 300 | Quality Level 100 | Quality Level - | Quality Level 200 |
| form liquid | form liquid | form liquid | form aqueous solution |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
Application
El sustrato TMB se utiliza en procedimientos ELISA en los que se utilizan conjugados de peroxidasa de rábano picante. El sustrato TMB desarrolla un producto de reacción azul soluble que puede leerse a 370 o 655 nm. La reacción se detiene con ácido, y se forma un producto de reacción amarillo que permite una medición precisa de la intensidad a 450 nm.
Cómo utilizar nuestro sustrato TMB:
Cómo utilizar nuestro sustrato TMB:
- Nuestro sustrato TMB se suministra como sustrato de peroxidasa listo para usar que contiene TMB en un tampón ligeramente ácido. Antes de la reacción con la peroxidasa, el sustrato debe ser una disolución de incolora a verde azulada clara. El sistema sustrato desarrolla un producto de reacción azul cuando reacciona con la peroxidasa.
- Una vez alcanzado el punto final del ensayo con la adición de ácido, se mide la intensidad del sustrato TMB a 450 nm.
- Nuestro sustrato TMB está recomendado para los procedimientos ELISA (micropocillo) y se ha utilizado en diversos ensayos como sustrato del conjugado de anticuerpos y peroxidasa de rábano picante para la detección de la glucoproteína del virus Hendra, la tetracosáctida, la inmunoglobulina A secretora (sIgA) y las trampas extracelulares de neutrófilos (NET).
- El sistema de sustrato líquido de 3,3′,5,5′-tetrametilbencidina (TMB) no se recomienda para aplicaciones de membrana. Dado que el sustrato TMB rinde un producto de reacción soluble, no se recomienda para histoquímica ni para inmunotransferencia.
General description
La TMB es un sustrato cromógeno de la peroxidasa de rábano picante - HRP.
Related product
Referencia del producto
Descripción
Precios
Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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I want to know about T0440 product concentration
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Helpful?
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How long should I develop? And what is the ideal OD between .1 and 1.0?
1 answer-
There is no set time at which the reaction must be stopped. Depending on the peroxidase activity measured, the color may form very quickly or slowly. Kindly adhere to the instructions provided in our datasheet as closely as possible.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/277/866/t0440dat-ms.pdfHelpful?
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What is the limit of detection for Product No. T0440, the 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System used in ELISA?
1 answer-
The limit of detection for Product No. T0440, the 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System used in ELISA, is specified to range from 3 to 6 pg/ml.
Helpful?
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What is the concentration of the TMB and what is the solvent in Product T0440, 3, 3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA?
1 answer-
The concentration of TMB in this formulation and the identity of the solvent are considered proprietary information. The product is a ready-to-use peroxidase substrate containing TMB in a mildly acidic buffer.
Helpful?
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Do I need to dilute Product T0440, 3, 3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA?
1 answer-
No, the product should not be diluted.. The product is ready-to-use for ELISA applications. If the intensity of the reaction needs to be reduced, it is recommended that the antibodies or conjugates be diluted. Usage information is described on our data sheet.
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What is the difference between products T0440, 3, 3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA, and T8665, 3,3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System?
1 answer-
Both products are comparable and either one can be used in ELISA procedures. The formulations which are proprietary may have some slight differences.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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Do I need to add hydrogen peroxide to Product T0440, 3, 3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA?
1 answer-
No hydrogen peroxide should be added to product T0440. The product, which contains a peroxidase substrate, already contains hydrogen peroxide. This solution is ready to use for ELISA assays.
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