Merck
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T0678

Sigma-Aldrich

Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse

clone WH-3, ascites fluid

Synonym(s):
Anti-TPH
Número MDL:
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

200

conjugado

unconjugated

forma del anticuerpo

ascites fluid

antibody product type

primary antibodies

clon

WH-3, monoclonal

mol peso

antigen 55 kDa

contiene

15 mM sodium azide

species reactivity

monkey, rat, human, rabbit

technique(s)

immunohistochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:1,000 using rabbit pineal gland extract

isotipo

IgG3

Nº de acceso UniProt

enviado en

dry ice

temp. de almacenamiento

−20°C

Gene Information

Descripción general

Monoclonal Anti-Tryptophan Hydroxylase (mouse IgG3 isotype) is derived from the WH-3 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Tryptophan hydroxylase (TPH; L-tryptophan tetrahydropteridine:oxygen oxidoreductase; EC 1.14.16.4), the rate-limiting enzyme for the synthesis of serotonin, is a very specific enzyme found only inside serotoninergic neurons.
The tryptophan hydroxylase 1 gene (TPH1) is a 444 amino acid protein encoded by the gene spanning 29 kbp with11 exons on human chromosome 11p15.3-p14. The enzyme is expressed in the gut, spleen, thymus, pituitary and also in the pineal gland.

Especificidad

This antibody reacts specifically with tryptophan hydroxylase.

Inmunógeno

recombinant rabbit tryptophan hydroxylase.

Aplicación

Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • immunohistochemistry
  • immunoblot
  • immunocytochemistry

Acciones bioquímicas o fisiológicas

The tryptophan hydroxylase 1 gene (TPH1) catalyzes the synthesis of 5-hydroxytryptophan, which is a precursor to the neurotransmitter serotonin. Variation in the gene expression affects prefrontal cortex activation during response inhibition. Mutation in the TPH1 gene leads to schizophrenia, irritable bowel syndrome (IBS) in women, suicidal behavior and depressive disorders. The encoded protein plays a vital role in regulation of cardiovascular function.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

nwg

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

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Certificate of Origin

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