Anti-β-Tubulin III antibody produced in rabbit

enhanced validation

affinity isolated antibody, buffered aqueous solution

Anti-Tuj 1
MDL number:
En este momento no podemos mostrarle ni los precios ni la disponibilidad

biological source


Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies




buffered aqueous solution

mol wt

antigen ~55 kDa

species reactivity

human, rat, mouse

enhanced validation

Learn more about Antibody Enhanced Validation


immunoprecipitation (IP): 10 μg using RIPA extract (250 μg) of cultured rat PC12 cells
indirect immunofluorescence: 10-20 μg/mL using rat PC12 cells
western blot: 0.2-0.4 μg/mL using whole extracts of mouse brain or cultured human neuroblastoma SH-SY5Y cells



UniProt accession no.

shipped in

dry ice

storage temp.


Gene Information

human ... TUBB3(10381)
mouse ... Tubb3(22152)
rat ... Tubb3(246118)

¿Está buscando productos similares? Visit Guía de comparación de productos

Categorías relacionadas

General description

Tubulin β 3 class III (TUBB3) also known as β-Tubulin III, is encoded by the gene mapped to human chromosome 16q24.3. TUBB3 protein expression is restricted to neurons.
α/β-Tubulin, an integral component of microtubules, is present in almost all eukaryotic cells.
α/β-Tubulin occurs mostly as soluble (approx. 100-110 kDa) heterodimeric sets of α- and β-tubulin isotypes or as polymers in assembled microtubules. β-Tubulin III (also designated β-4 chain) is found in the brain and dorsal root ganglia and appears to be localized to neurons of the central and peripheral nervous system. β-tubulin III is also found in Sertoli cells of the testis, spermatozoa tails, certain lung cells, and apparently some breast stromal cells.


Anti-β-Tubulin III recognizes human, mouse,and rat β-tubulin III.


synthetic peptide corresponding to amino acid residues 441-450 of human β-tubulin III (Ala446 to Ser446 substitution) with N-terminal added cysteine, conjugated to KLH. The sequence is conserved in mammals.


Anti-β-Tubulin III antibody produced in rabbit has been used in following studies:
  • Immunofluorescence
  • Immunostaining
  • Western blotting
Anti-β-Tubulin III antibody produced in rabbit has been used in following studies:
  • immunofluorescence
  • immunostaining
  • western blotting
  • indirect immunoperoxidase assay (IPA)

Biochem/physiol Actions

Tubulin β 3 class III (TUBB3) plays a vital role in nervous system development and axon guidance. Mutation of the gene encoding protein leads to the ocular motility disorder, congenital fibrosis of the extraocular muscle type 3 (CFEOM3), and various neurological syndromes. Altered expression of the protein affects microtubule dynamics and microtubule-kinesin interactions. Increased expression of TUBB3 is observed in tumor tissues. Decreased expression of TUBB3 can be considered as an important marker for poor prognosis of cutaneous malignant melanoma. TUBB3 is implicated in the suppression of invasive growth of tumor tissue;therefore, it is considered to be a potential target for development of antitumor drugs.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Storage and Stability: For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Other Notes

In order to obtain the best results using various techniques and preparations, we recommend determining the optimal working concentrations by titration.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


NONH for all modes of transport

WGK Germany


Flash Point F

Not applicable

Flash Point C

Not applicable

Certificado de Análisis
Geometric effect of cell adhesive polygonal micropatterns on neuritogenesis and axon guidance.
Jang MJ, Nam Y.
Journal of Neural Engineering, 9(4) (2012)
Reciprocal regulation of the basic helix-loop-helix/Per-Arnt-Sim partner proteins, Arnt and Arnt2, during neuronal differentiation.
Hao N
Nucleic Acids Research, 41(11), 5626-5638 (2013)
Immunohistochemical, volumetric, and functional neuroimaging studies in patients with idiopathic Parkinson's disease.
Hummel T, et al.
Journal of the Neurological Sciences, 289(1-2), 119-122 (2010)
Tubulin beta 3 and 4 are involved in the generation of early fibrotic stages.
Wawro M E, et al.
Cellular Signalling, 38, 26-38 (2017)
Characterization of the colchicine binding site on avian tubulin isotype βVI.
Sharma S, et al.
Biochemistry, 49(13), 2932-2942 (2010)

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico

Redes sociales

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon


Investigación. Desarrollo. Producción.

Somos un proveedor líder para la industria de Ciencias de la Vida con soluciones y servicios para investigación, desarrollo y producción biotecnológicos, y para desarrollo y producción de tratamientos farmacéuticos

© 2021 Merck KGaA, Darmstadt, Alemania y/o sus filiales. Todos los derechos reservados.

Queda estrictamente prohibida la reproducción sin permiso de cualquiera de los materiales de la página web.