Merck

T2200

Sigma-Aldrich

Anti-β-Tubulin III antibody produced in rabbit

enhanced validation

affinity isolated antibody, buffered aqueous solution

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Sinónimos:
Anti-Tuj 1
Número MDL:
NACRES:
NA.41

origen biológico

rabbit

conjugado

unconjugated

forma del anticuerpo

affinity isolated antibody

antibody product type

primary antibodies

clon

polyclonal

formulario

buffered aqueous solution

mol peso

antigen ~55 kDa

species reactivity

rat, human, mouse

validación mejorada

knockout
Learn more about Antibody Enhanced Validation

technique(s)

immunoprecipitation (IP): 10 μg using RIPA extract (250 μg) of cultured rat PC12 cells
indirect immunofluorescence: 10-20 μg/mL using rat PC12 cells
western blot: 0.2-0.4 μg/mL using whole extracts of mouse brain or cultured human neuroblastoma SH-SY5Y cells

Nº de acceso UniProt

enviado en

dry ice

temp. de almacenamiento

−20°C

target post-translational modification

unmodified

Gene Information

human ... TUBB3(10381)
mouse ... Tubb3(22152)
rat ... Tubb3(246118)

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Este artículo
T8578T8660T5076
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

purified immunoglobulin

clone

polyclonal

clone

2G10, monoclonal

clone

SDL.3D10, monoclonal

clone

SDL.3D10, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

-

form

buffered aqueous solution

mol wt

antigen ~55 kDa

mol wt

antigen ~50 kDa

mol wt

-

mol wt

-

Descripción general

Tubulin β 3 class III (TUBB3) also known as β-Tubulin III, is encoded by the gene mapped to human chromosome 16q24.3. TUBB3 protein expression is restricted to neurons.
α/β-Tubulin, an integral component of microtubules, is present in almost all eukaryotic cells.
α/β-Tubulin occurs mostly as soluble (approx. 100-110 kDa) heterodimeric sets of α- and β-tubulin isotypes or as polymers in assembled microtubules. β-Tubulin III (also designated β-4 chain) is found in the brain and dorsal root ganglia and appears to be localized to neurons of the central and peripheral nervous system. β-tubulin III is also found in Sertoli cells of the testis, spermatozoa tails, certain lung cells, and apparently some breast stromal cells.

Especificidad

Anti-β-Tubulin III recognizes human, mouse,and rat β-tubulin III.

Inmunógeno

synthetic peptide corresponding to amino acid residues 441-450 of human β-tubulin III (Ala446 to Ser446 substitution) with N-terminal added cysteine, conjugated to KLH. The sequence is conserved in mammals.

Aplicación

Anti-β-Tubulin III antibody produced in rabbit has been used in following studies:
  • Immunofluorescence
  • Immunostaining
  • Western blotting
Anti-β-Tubulin III antibody produced in rabbit has been used in following studies:
  • immunofluorescence
  • immunostaining
  • western blotting
  • indirect immunoperoxidase assay (IPA)

Acciones bioquímicas o fisiológicas

Tubulin β 3 class III (TUBB3) plays a vital role in nervous system development and axon guidance. Mutation of the gene encoding protein leads to the ocular motility disorder, congenital fibrosis of the extraocular muscle type 3 (CFEOM3), and various neurological syndromes. Altered expression of the protein affects microtubule dynamics and microtubule-kinesin interactions. Increased expression of TUBB3 is observed in tumor tissues. Decreased expression of TUBB3 can be considered as an important marker for poor prognosis of cutaneous malignant melanoma. TUBB3 is implicated in the suppression of invasive growth of tumor tissue;therefore, it is considered to be a potential target for development of antitumor drugs.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Nota de preparación

Storage and Stability: For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Otras notas

In order to obtain the best results using various techniques and preparations, we recommend determining the optimal working concentrations by titration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

producto relacionado

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable


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Geometric effect of cell adhesive polygonal micropatterns on neuritogenesis and axon guidance.
Jang MJ, Nam Y.
Journal of Neural Engineering, 9(4) (2012)
Chikungunya virus nonstructural protein 2 inhibits type I/II interferon-stimulated JAK-STAT signaling.
Fros JJ
Journal of Virology, 84(20), 10877-10887 (2010)
Characterization of the colchicine binding site on avian tubulin isotype βVI.
Sharma S, et al.
Biochemistry, 49(13), 2932-2942 (2010)
Reciprocal regulation of the basic helix-loop-helix/Per-Arnt-Sim partner proteins, Arnt and Arnt2, during neuronal differentiation.
Hao N
Nucleic Acids Research, 41(11), 5626-5638 (2013)
Immunohistochemical, volumetric, and functional neuroimaging studies in patients with idiopathic Parkinson's disease.
Hummel T, et al.
Journal of the Neurological Sciences, 289(1-2), 119-122 (2010)

Protocolos

Protocol Guide: Differentiation of Human Neural Stem Cells using TrueGel3D® HTS Hydrogel Plates

Learn to culture ReNcell® human neural stem cells (NSCs) in 3D hydrogels like the TrueGel3D® HTS Hydrogel Plate using this step by step protocol for high-throughput screening cell culture applications.

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