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T3198

Sigma-Aldrich

Monoclonal Anti-Tumor Necrosis Factor-α antibody produced in mouse

clone 45418.111, purified immunoglobulin, lyophilized powder

Synonym(s):
Anti-TNF-α
Número MDL:
NACRES:
NA.41

Nivel de calidad

100

origen biológico

mouse

conjugado

unconjugated

forma del anticuerpo

purified immunoglobulin

antibody product type

primary antibodies

clon

45418.111, monoclonal

formulario

lyophilized powder

species reactivity

rat

technique(s)

capture ELISA: suitable
neutralization: suitable
western blot: 1-2 μg/mL

isotipo

IgG1

Nº de acceso UniProt

temp. de almacenamiento

−20°C

Gene Information

rat ... Tnf(24835)

Descripción general

The antibody has the ability to neutralize the biological activity of rat TNF-α.and may also be used as a capture antibody in ELISAs.

Inmunógeno

purified recombinant rat TNF-α expressed in E. coli.

Aplicación

Monoclonal Anti-Tumor necrosis factor-α antibody produced in mouse has been used in immunoneutralization study and western blot analysis.

Acciones bioquímicas o fisiológicas

Tumor Necrosis Factor-alpha (TNF-α) is a cytokine produced by macrophages in response to stimulus by LPS. It plays a major role in mediating inflammation, tissue injury, pathogenic shock, innate immunity, apoptosis and autoimmunity. The physiological effects of TNF-α are mediated by receptors of Tumor necrosis factor receptor (TNFR) super family. TNF/TNFR also results in the activation of downstream pathways involving mitogen-activated protein kinase (MAPK), p38(a class of MAPK), c-Jun N-terminal kinase (JNK) and NF-κB (Nuclear Factor kappa-light-chain-enhancer of activated B cells) that play a major role in innate immunity, acute inflammatory responses and homeostasis.
Tumor necrosis factor-α (TNFα) affects various cellular processes such as blood-brain barrier, inflammatory, thrombogenic and vascular changes following brain damage. TNFα expression is upregulated in various central nervous system disorders, including Alzheimer′s disease, multiple sclerosis, Parkinson′s disease, meningococcal meningitis and human immunodeficiency virus (HIV) infection. Elevated expression of TNFα in the brain and blood in response to lipopolysaccharide might increase brain stem thrombosis, hemorrhage and stroke sensitivity/risk in hypertensive rats. Tumor necrosis factor-α activates polymorphonuclear leukocyte NADPH oxidase, thereby facilitates development of systemic oxidative stress (OS), inflammation and hypertension in rats.

Forma física

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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Quotes and Ordering

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