Merck
All Photos(5)

T3253

Sigma-Aldrich

Trizma® hydrochloride

reagent grade, ≥99.0% (titration), crystalline

Synonym(s):
TRIS HCl, Tris(hydroxymethyl)aminomethane hydrochloride, TRIS hydrochloride, Tromethane hydrochloride
Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
Molecular Weight:
157.60
Beilstein:
3675235
Número de EC:
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.25

Nivel de calidad

400

grado

reagent grade

ensayo

≥99.0% (titration)

formulario

crystalline

impurezas

≤0.5% water (Karl Fischer)

useful pH range

7.0-9.0

pKa (25 °C)

8.1

mp

150-152 °C

solubilidad

H2O: soluble, clear, colorless

absorción

≤0.05 at 290 nm at 40%

application(s)

diagnostic assay manufacturing

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Descripción general

Trizma® hydrochloride is mainly required for preparation of buffer at physiological range of 7.3 to 7.5. The prepared buffer is compatible with biological fluids. It is of importance to laboratories as a standard pH solution.

Aplicación

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Trizma® hydrochloride has been used as a buffer solution for staining procedure, reduction and alkylation studies and in situ hybridization procedure.­

Envase

100, 250, 500 g in poly bottle
5, 10, 25, 50 kg in poly drum
1 kg in poly bottle

Otras notas

Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.

Información legal

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Protocols

Hand-casting gels for PAGE and SDS-PAGE using TurboMix® Bis-Tris Gel Casting Kits

Overview of polyacrylamide gel chemistry and detailed instructions for hand-casting polyacrylamide gels using TurboMix® Bis-Tris Gel Casting Kits

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service