Merck
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T3320

Sigma-Aldrich

Anti-γ-Tubulin (AK-15) antibody produced in rabbit

enhanced validation

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):
Anti-TUBGCP1, Anti-CDCBM4, Anti-GCP-1, Anti-TUBG
Número MDL:

origen biológico

rabbit

Nivel de calidad

200

conjugado

unconjugated

forma del anticuerpo

IgG fraction of antiserum

antibody product type

primary antibodies

clon

polyclonal

formulario

buffered aqueous solution

mol peso

antigen 48 kDa

species reactivity

rat, human, frog

validación mejorada

independent
Learn more about Antibody Enhanced Validation

technique(s)

immunocytochemistry: 1:1,000 using methanol-acetone fixed, cultured rat, RAT-2 cells
microarray: suitable
western blot: 1:1,000 using whole cell extract of the human epitheloid carcinoma HeLa cell line.
western blot: 1:5,000 using Xenopus eggs extract

Nº de acceso UniProt

enviado en

dry ice

temp. de almacenamiento

−20°C

Gene Information

Descripción general

γ-Tubulin (48 kDa) is a highly conserved protein. The microtubule cytoskeleton consists of a dynamic, highly polarized network of microtubules filaments, microtubule-associated proteins, microtubule motors, and microtubule-organizing proteins. γ-Tubulin is found as part of a large protein complex containing at least five other proteins and has a shape of a ring (γ-tubulin ring complex, γ-TuRC) that is roughly the same diameter as a microtubule.
γ-Tubulin, mapped on human chromosome 17q21.2, codes for a member of the tubulin family. It is localized to the microtubule organizing centres. γ -Tubulin consists of two isoforms tubulinγ 1 (TUBG1) and tubulin γ 2 (TUBG2) with 97.3% amino acid identity. In addition to these two isoforms, γ-tubulin pseudogene (TUBG1P) is also been identified on human chromosome 17. Human TUBG1 and TUBG2 transcripts are widely expressed in preimplantation embryos and the brain, respectively. γ -tubulin is a component of γ-tubulin ring complex (γ-TuRC), which has roughly the same diameter as a microtubule.

Aplicación

Anti-γ-Tubulin (AK-15) antibody produced in rabbit has been used in:
  • western blotting
  • immunocytochemistry (immunofluorescence staining
  • immunofluorescence

Anti-γ-Tubulin (AK-15) antibody produced in rabbit has been used in following studies:
  • Western blot.
  • Immunofluorescence.

Acciones bioquímicas o fisiológicas

γ-Tubulin nucleates microtubule assembly throughout the mammalian cell cycle in vivo. In Aspergillus nidulans, γ-tubulin facilitates attachment of microtubules to the spindle pole body, nuclear division and microtubule assembly. Ubiquitination of γ-tubulin by breast cancer 1 protein (BRCA1) is a crucial step in the regulation of centrosome number. Overexpression ofγ-tubulin is observed in lung cancer. Tubulin γ 2 (TUBG2) plays a vital role in cell growth. Aberration in the γ-tubulin gene alters microtubule assembly. The expression levels of γ-tubulin can be considered as an important prognostic indicator for patients with astrocytomas.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

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Certificate of Origin

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Quotes and Ordering

Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

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