γ-Tubulin (48 kDa) is a highly conserved protein. The microtubule cytoskeleton consists of a dynamic, highly polarized network of microtubules filaments, microtubule-associated proteins, microtubule motors, and microtubule-organizing proteins. γ-Tubulin is found as part of a large protein complex containing at least five other proteins and has a shape of a ring (γ-tubulin ring complex, γ-TuRC) that is roughly the same diameter as a microtubule.
γ-Tubulin, mapped on human chromosome 17q21.2, codes for a member of the tubulin family. It is localized to the microtubule organizing centres. γ -Tubulin consists of two isoforms tubulinγ 1 (TUBG1) and tubulin γ 2 (TUBG2) with 97.3% amino acid identity. In addition to these two isoforms, γ-tubulin pseudogene (TUBG1P) is also been identified on human chromosome 17. Human TUBG1 and TUBG2 transcripts are widely expressed in preimplantation embryos and the brain, respectively. γ -tubulin is a component of γ-tubulin ring complex (γ-TuRC), which has roughly the same diameter as a microtubule.
Anti-γ-Tubulin (AK-15) antibody produced in rabbit has been used in:
- western blotting
- immunocytochemistry (immunofluorescence staining
Anti-γ-Tubulin (AK-15) antibody produced in rabbit has been used in following studies:
- Western blot.
Acciones bioquímicas o fisiológicas
γ-Tubulin nucleates microtubule assembly throughout the mammalian cell cycle in vivo. In Aspergillus nidulans, γ-tubulin facilitates attachment of microtubules to the spindle pole body, nuclear division and microtubule assembly. Ubiquitination of γ-tubulin by breast cancer 1 protein (BRCA1) is a crucial step in the regulation of centrosome number. Overexpression ofγ-tubulin is observed in lung cancer. Tubulin γ 2 (TUBG2) plays a vital role in cell growth. Aberration in the γ-tubulin gene alters microtubule assembly. The expression levels of γ-tubulin can be considered as an important prognostic indicator for patients with astrocytomas.
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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