Hybridization of genomic DNA to oligonucleotide probes in the presence of tetramethylammonium chloride.
A G DiLella et al.
Methods in enzymology, 152, 447-451 (1987-01-01)
W B Melchior et al.
Proceedings of the National Academy of Sciences of the United States of America, 70(2), 298-302 (1973-02-01)
Several small alkylammonium ions can eliminate, or even reverse, the usual dependence of the DNA transition temperature on base composition. For example, in 3 M tetramethylammonium chloride, or 2.4 M tetraethylammonium chloride, DNAs of different base compositions all melt at...
Markus Welcker et al.
Genes & development, 27(23), 2531-2536 (2013-12-04)
The Fbw7 tumor suppressor targets a broad network of proteins for ubiquitylation. Here we show critical functions for Fbw7 dimerization in regulating the specificity and robustness of degradation. Dimerization enables Fbw7 to target substrates through concerted binding to two suboptimal...
Lulu Xie et al.
Science (New York, N.Y.), 342(6156), 373-377 (2013-10-19)
The conservation of sleep across all animal species suggests that sleep serves a vital function. We here report that sleep has a critical function in ensuring metabolic homeostasis. Using real-time assessments of tetramethylammonium diffusion and two-photon imaging in live mice...
Wei Shen et al.
Chemical communications (Cambridge, England), 49(73), 8114-8116 (2013-08-08)
A simple and low-cost colorimetric assay utilizing ferrofluidic nanoparticulate probes (FNPs) and a ligase for single-nucleotide polymorphism genotyping is described. Excellent sensitivity and selectivity were accomplished through the engagement of the FNPs and a ligase chain reaction.