T3559

Anti-γ-Tubulin antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Sinónimos: Anti-γ-Tubulin
• NACRES: NA.46

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: IgG fraction of antiserum
• antibody product type: primary antibodies
• clon: polyclonal
• formulario: buffered aqueous solution
• mol peso: antigen 48 kDa
• species reactivity: chicken, human
• envase: antibody small pack of 25 μL
• validación mejorada: independent; Learn more about Antibody Enhanced Validation
• technique(s): indirect immunofluorescence: 1:5,000 using methanol/acetone-fixed chicken fibroblasts; microarray: suitable; western blot: 1:1,000 using cultured A431 whole cell extracts
• Nº de acceso UniProt: P23258
• enviado en: dry ice
• temp. de almacenamiento: −20°C
• Gene Information: human ... TUBG1(7283) , TUBG2(27175)

Descripción

Descripción general

γ-Tubulin (48kDa) is a ubiquitous and highly conserved protein within the microtubule organizing centers (MTOCs) in eukaryotic cells. It is related to α- and β-tubulin and is a member of the tubulin superfamily of proteins. γ-Tubulin abundance is less than 1% of the level of either α- or β-tubulin. γ-Tubulin shares approximately 28-32% identity with α-tubulin from various organisms and 32-36% identity with β-tubulins. Some regions are highly conserved among α-, β- and γ-tubulins.

Especificidad

Anti-gamma-Tubulin recognizes an epitope located in the N-terminal region of gamma-tubulin (amino acids 38-53). Staining of gamma-tubulin (48 kDa) by immunoblotting is specifically inhibited with gamma-tubulin peptide (human, amino acids 38-53 with C-terminally added lysine).

Inmunógeno

synthetic peptide corresponding to the N-terminal region of human γ-tubulin (amino acids 38-53, with C-terminally added lysine) conjugated to KLH. This sequence is specific for γ-tubulin and not found in other members of the tubulin family such as α− β−, δ and ε− tubulins. This sequence is identical in mouse and rat γ-tubulin and highly conserved among species (Drosophila, Aspergillus, and yeast γ-tubulin).

Aplicación

Anti-γ-Tubulin antibody produced in rabbit has been used in

• immunofluorescence analysis
• western blotting
• immunohistochemistry

Anti-γ-Tubulin polyclonal antibody is suitable for immunoblotting of materials such as A431 cultured cells whole extract (g-tubulin, 48 kDa), and immunocytochemical staining of cells such as cultured chicken fibroblast cells.

Acciones bioquímicas o fisiológicas

γ-Tubulin binds microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome. γ-Tubulin is not a member of microtubule like α- and β-tubulin. It functions as the microtubule nucleator at the microtubule organizing centre (MTOC). It binds to the β-tubulin half of the tubulin molecule, thus establishing the polarity of a microtubule, leaving the α-tubulin half exposed at the plus end.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• WGK: WGK 3
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable