Merck
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T5941

Sigma-Aldrich

Trizma® hydrochloride

BioPerformance Certified, suitable for cell culture, ≥99.0% (titration)

Synonym(s):
TRIS HCl, Tris(hydroxymethyl)aminomethane hydrochloride, TRIS hydrochloride, Tromethane hydrochloride
Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
Molecular Weight:
157.60
Beilstein:
3675235
Número de EC:
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.25

grado

BioPerformance Certified

Nivel de calidad

400

ensayo

≥99.0% (titration)

formulario

crystalline powder

technique(s)

cell culture | mammalian: suitable
electrophoresis: suitable

impurezas

DNAse, Exonuclease; NICKase, Endonuclease; RNAse and Protease, none detected
endotoxin and total aerobic microbial count, tested
≤0.5% water (Karl Fischer)

useful pH range

7.0-9.0

pKa (25 °C)

8.1

mp

150-152 °C

solubilidad

H2O: 667 mg/mL

absorción

≤0.05 at 290 at 40%

idoneidad

suitable for electrophoresis

application(s)

diagnostic assay manufacturing

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

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Aplicación

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Envase

1 kg in poly bottle
100, 500 g in poly bottle
5 kg in poly drum
T5941-1KT:
Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.

Otras notas

Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.

Información legal

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Protocols

Plasmid DNA Preparation

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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