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T6146

Sigma-Aldrich

Trypan Blue

powder, BioReagent, suitable for cell culture

Sinónimos:
Direct blue 14
Fórmula lineal:
C34H24N6O14S4Na4
Número de CAS:
Peso molecular:
960.81
Número de índice de color:
23850
Beilstein:
4360496
Número de EC:
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.75

Nivel de calidad

200

línea de producto

BioReagent

formulario

powder

composición

Dye content, ~40%

envase

glass bottle of 100 g
glass bottle of 25 g
glass bottle of 5 g

condiciones de almacenamiento

dry at room temperature

technique(s)

cell culture | mammalian: suitable
tissue processing: suitable

mp

>300 °C (lit.)

solubilidad

H2O: 10 mg/mL

application(s)

cell analysis

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

Gene Information

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Descripción general

The trypan blue dye is an azo dye, derived from toluidine. It is soluble in water and has a molecular weight of 961 g/mol.

Aplicación

Trypan blue has been used:
  • in cell viability assay
  • to determine the number of living and necrotic cells by staining with 0.2% trypan blue solution.
  • to stain plant tissue in order to to visualize cell death

Envase

5, 25, 100 g in glass bottle

Acciones bioquímicas o fisiológicas

The trypan blue dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.

pictogramas

Health hazard

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Carc. 1B

Código de clase de almacenamiento

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Equipo de protección personal

Eyeshields, Gloves, type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges

Certificado de Análisis

Certificado de origen

Plant tissue trypan blue staining during phytopathogen infection.
Fernandez NB, et al.
Bio-protocol (2016)
Acanthamoeba: Biology and Pathogenesis, 85-85 (2009)
Trypan blue exclusion test of cell viability
Strober W
Current Protocols in Immunology, 21(1), A-3B (1997)
Knockdown of PSMB7 induces autophagy in cardiomyocyte cultures: possible role in endoplasmic reticulum stress
Kyrychenko VO. et al.
Pathobiology, 81(1), 8-14 (2014)
A histological analysis of lens capsules stained with trypan blue for capsulorrhexis in phacoemulsification cataract surgery
Singh AJ, et al.
Eye (London, England), 17(5), 567-567 (2003)

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