Merck
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T6567

Sigma-Aldrich

Trypsin from porcine pancreas

Proteomics Grade, BioReagent, Dimethylated

Synonym(s):
Porcine Trypsin, Trypsin for Mass Spectropetry
Número de EC:
Número MDL:
NACRES:
NA.56

origen biológico

Porcine pancreas

Nivel de calidad

Línea del producto

BioReagent

solubilidad

1 mM HCl: soluble 1 mg/mL, clear, colorless

enviado en

wet ice

temp. de almacenamiento

2-8°C

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This Item
1.02307SRP6509A3176
biological source

Porcine pancreas

biological source

-

biological source

human

biological source

Porcine pancreas

solubility

1 mM HCl: soluble 1 mg/mL, clear, colorless

solubility

-

solubility

-

solubility

-

shipped in

wet ice

shipped in

-

shipped in

wet ice

shipped in

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

-

Quality Level

-

Quality Level

200

product line

BioReagent

product line

-

product line

-

product line

-

Descripción general

Trypsin is a serine protease that is usually used in biochemistry and biology as an important enzymatic reagent. This method produces a highly purified trypsin product suitable for proteomics research. Proteomics Grade ideal for use in both solution and in-gel tryptic digestions. Trypsin, a serine protease, is present in the digestive system of several vertebrates.

Aplicación

Trypsin from porcine pancreas is used for the following applications:
  • In-gel protein digestion and MALDI-TOF mass spectrometry analysis
  • Electrospray Ionization Mass Spectrometry (ESI-MS) analysis
  • Surface proteome profiling of L. plantarum
  • Gel Filtration, Ultracentrifugation, and Rotary Shadowing Electron Microscopy
  • Mass spectrometry

Acciones bioquímicas o fisiológicas

Trypsin is routinely used in proteomics research for peptide mapping and protein sequence work, due to its highly specific cleavage resulting in a limited number of tryptic peptides. It hydrolyzes peptide bonds specifically at the carboxyl side of arginine and lysine residues.. The enzyme also exhibits esterase and amidase activities. Trypsin acts as a cell culture tool. It is used to hydrolyze allergenic proteins to produce hypoallergenic milk in industries.

pictogramas

Exclamation markHealth hazard

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 1

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificate of Analysis

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Certificate of Origin

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