Merck
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T7193

Sigma-Aldrich

Trizma® Pre-set crystals

BioPerformance Certified, pH 7.0, average Mw 154.8

Synonym(s):
Tris crystals
NACRES:
NA.25

Nivel de calidad

200

grado

BioPerformance Certified

formulario

crystalline powder

mol peso

average Mw 154.8

technique(s)

cell culture | mammalian: suitable

impurezas

DNase, RNase, Protease, NICKase, none detected
endotoxin, tested
total aerobic microbial count, tested
≤0.05 A290
≤0.5% water (Karl Fischer)
≤5 ppm Heavy metals (as Pb)

pH

7.00 (25 °C, 0.1 M)(lit.)
7.0

useful pH range

7.0-9.0

solubilidad

water: 0.667 g/mL, clear, colorless

application(s)

diagnostic assay manufacturing

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Aplicación

Dissolve in water to make a 0.1M solution. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.

Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.

If a different concentration is desired, note that there is a concentration effect on the pH of the solution.
The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases.

Envase

100, 250 g in poly bottle

Otras notas

This product is a pre-mixed combination of Trizma base and Trizma HCl that can be used to prepare a common pH value for Tris buffer.
Certain pH electrodes do not give accurate results with Tris buffers.

Nota de preparación

Trizma solutions can be autoclaved.

Información legal

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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