Merck
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T7539

Sigma-Aldrich

Tumor Necrosis Factor-α from mouse

TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture

Synonym(s):
mTNF-α, TNF-α
Número MDL:
NACRES:
NA.77

Nivel de calidad

200

origen biológico

mouse

recombinante

expressed in E. coli

ensayo

≥95%

formulario

powder

potencia

0.01-0.5 ng/mL EC50

mol peso

17 kDa

envase

pkg of 10 μg
pkg of 50 μg

condiciones de almacenamiento

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurezas

≤1 EU/μg protein endotoxin

color

white

solubilidad

water: soluble

Nº de acceso UniProt

temp. de almacenamiento

−20°C

Gene Information

mouse ... Tnf(21926)

Aplicación

Tumor Necrosis Factor-α from mouse, a potent proinflammatory cytokine has been used:
  • to activate MS1 endothelium cells
  • to induce lipolysis in adipocytes
  • to study its effect on liganded and unliganded glucocorticoid receptor (GR) in interleukin (IL)-6 and IL-8 gene regulation

Acciones bioquímicas o fisiológicas

Tumor necrosis factor-α (TNF-α), also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is expressed by a great variety of cells, with numerous inductive and suppressive agents. Primarily, TNF-α is produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. Although TNF-α is used in clinical trials as an antitumor agent, Sigma′s cytokine, growth factor and hormone products are for research only. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2. Mouse and human TNF-α share 79% amino acid sequence identity. Unlike human TNF-α, the mouse form is glycosylated.

Definición de unidad

One unit is the amount required to induce half-maximal cytolysis of L929 cells.

Forma física

Lyophilized from 10 mM sodium phosphate, pH 7.5

pictogramas

Exclamation mark

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Eye Irrit. 2 - Skin Irrit. 2

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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