T7539

Sigma-Aldrich

Tumor Necrosis Factor-α from mouse

TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture

Sinónimos:
mTNF-α, TNF-α
MDL number:
NACRES:
NA.77
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Quality Level

biological source

mouse

recombinant

expressed in E. coli

assay

≥95%

form

powder

potency

0.01-0.5 ng/mL EC50

mol wt

17 kDa

packaging

pkg of 10 μg
pkg of 50 μg

storage condition

avoid repeated freeze/thaw cycles

application(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg protein endotoxin

color

white

solubility

water: soluble

UniProt accession no.

storage temp.

−20°C

Gene Information

mouse ... Tnf(21926)

Categorías relacionadas

Application

Tumor Necrosis Factor-α from mouse, a potent proinflammatory cytokine has been used:
  • to activate MS1 endothelium cells
  • to induce lipolysis in adipocytes
  • to study its effect on liganded and unliganded glucocorticoid receptor (GR) in interleukin (IL)-6 and IL-8 gene regulation

Biochem/physiol Actions

Tumor necrosis factor-α (TNF-α), also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is expressed by a great variety of cells, with numerous inductive and suppressive agents. Primarily, TNF-α is produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. Although TNF-α is used in clinical trials as an antitumor agent, Sigma′s cytokine, growth factor and hormone products are for research only. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2. Mouse and human TNF-α share 79% amino acid sequence identity. Unlike human TNF-α, the mouse form is glycosylated.

Unit Definition

One unit is the amount required to induce half-maximal cytolysis of L929 cells.

Physical form

Lyophilized from 10 mM sodium phosphate, pH 7.5

pictograms

Exclamation mark

signalword

Warning

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point F

Not applicable

Flash Point C

Not applicable

Glucocorticoid-independent repression of tumor necrosis factor (TNF) $\alpha$-stimulated interleukin (IL)-6 expression by the glucocorticoid receptor a potential mechanism for protection against an excessive inflammatory response
Verhoog NJD, et al.
The Journal of Biological Chemistry, 286(22), 19297-19310 (2011)
Relative contribution of adipose triglyceride lipase and hormone-sensitive lipase to tumor necrosis factor-$\alpha$ (TNF-$\alpha$)-induced lipolysis in adipocytes
Yang X, et al.
The Journal of Biological Chemistry, 286(47), 40477-40485 (2011)
SDF-1--CXCR4 differentially regulates autoimmune diabetogenic T cell adhesion through ROBO1--SLIT2 interactions in mice
Glawe JD, et al.
Diabetologia, 56(10), 2222-2230 (2013)
Jing Deng et al.
Molecular and cellular biology, 24(23), 10161-10168 (2004-11-16)
Numerous stressful conditions activate kinases that phosphorylate the alpha subunit of translation initiation factor 2 (eIF2alpha), thus attenuating mRNA translation and activating a gene expression program known as the integrated stress response. It has been noted that conditions associated with...
Matteo Cornaglia et al.
Analytical chemistry, 86(16), 8213-8223 (2014-07-30)
We describe the concept of magnetic particle-scanning for on-chip detection of biomolecules: a magnetic particle, carrying a low number of antigens (Ag's) (down to a single molecule), is transported by hydrodynamic forces and is subjected to successive stochastic reorientations in...
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