T7777

Sigma-Aldrich

Tris-Glycine-SDS Buffer 10× Concentrate

Sinónimos:
TRIS glycine SDS buffer solution
eCl@ss:
32129211
NACRES:
NA.25
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Quality Level

sterility

0.2 μm filtered

Featured Industry

Diagnostic Assay Manufacturing

storage temp.

2-8°C

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General description

Tris-glycine-SDS (TGS) running buffer is the most commonly used buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. TGS is usually used for both anode and cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units.

Application

Tris-glycine-SDS buffer 10× concentrate has been used as a running buffer in sodium dodecyl sulfate-polyacryamide gel electrophoresis (SDS-PAGE). It is also used to block membranes with 5% non-fat dry milk for western blotting. TGS is usually used for both the anode buffer and the cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units.

Reconstitution

Dilution of the 10× TGS buffer produces a 1× running buffer containing 25 mM Tris, 192 mM glycine and 0.1% SDS, pH approx. 8.6.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificado de Análisis
Multiple tandem epitope tagging for enhanced detection of protein expressed in mammalian cells
Zhang L, et al.
Molecular Biotechnology, 19(3), 313-321 (2001)
Antitumor Activity and Mechanism of a Reverse Transcriptase Inhibitor, Dapivirine, in Glioblastoma
Liu W, et al.
Journal of Cancer, 117?128-117?128 (2018)
A Radioisotope-free Oligosaccharyltransferase Assay Method
Yamasaki T and Kohda D
Glycobiology (2007)
Man-Lan Guo et al.
Cell biochemistry and function, 37(2), 72-83 (2019-02-19)
Cancer cell progression and proliferation increase cell density, resulting in changes to the tumour site, including the microenvironment. What is not known is if increased cell density influences the aggressiveness of cancer cells, especially their proliferation, migration, and invasion capabilities....
Weiwen Liu et al.
Journal of Cancer, 9(1), 117-128 (2018-01-02)
Dapivirine is one of reverse transcriptase inhibitors (RTIs). It is the prototype of diarylpyrimidines (DAPY), formerly known as TMC120 or DAPY R147681 (IUPAC name: 4- [[4-(2, 4, 6-trimethylphenyl) amino]-2-pyrimidinyl] amino]-benzonitrile; CAS no.244767-67-7). The purpose of this study is to investigate...

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