Merck
  • T7816
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T7816

Sigma-Aldrich

Monoclonal Anti-β-Tubulin I antibody produced in mouse

clone SAP.4G5, ascites fluid

Synonym(s):
Anti-CDCBM6, Anti-CSCSC1, Anti-M40, Anti-OK/SW-cl.56, Anti-TUBB1, Anti-TUBB5
Número MDL:
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

ascites fluid

antibody product type

primary antibodies

clon

SAP.4G5, monoclonal

mol peso

antigen ~55 kDa

species reactivity

Xenopus, chicken, human, hamster, canine, mouse, bovine, rat

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:20,000 using cultured chicken fibroblasts

isotipo

IgG1

Nº de acceso UniProt

enviado en

dry ice

temp. de almacenamiento

−20°C

Gene Information

human ... TUBB(203068)
mouse ... Tubb4(22153)

Descripción general

Tubulin a 100kD protein is present in all cells as a heterodimer of two similar polypeptides α and β which assemble to form microtubules. Proper organization of microtubules is essential for several cellular functions like mitosis, meiosis, some forms of organellar movement, intracellular transport, flagellar movement and cytoskeletal functions. Monoclonal anti-β-tubulin I antibody can be used in ELISA to localize β tubulin isotype I and can be used in immunoblotting to localize cultured human and animal cells. Mouse anti-β-tubulin I antibody reacts specifically with an epitope found in the c-terminal sequence of β tubulin of human, rat, dog, bovine, hamster, mouse, chicken and xenopus

Especificidad

Recognizes an epitope located in the C-terminal sequence of β-tubulin isotype I. No reactivity with other tubulin isotypes is observed.

Inmunógeno

synthetic peptide corresponding to the C-terminal sequence of β-tubulin isotype I, conjugated to BSA.

Aplicación

Monoclonal anti-β-tubulin I antibody (diluted 1: 4,000) can be used as a primary antibody in immunoprecipitation and immunoblotting. It can also be used in western blot analysis and retroviral infection.

Envase

100 μL
0.2 mL

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

nwg

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

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Certificate of Origin

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