T7824

Sigma-Aldrich

Anti-TAK1 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Sinónimos:
Anti-TGFβ Activated Kinase
MDL number:
NACRES:
NA.44
En este momento no podemos mostrarle ni los precios ni la disponibilidad

biological source

rabbit

Quality Level

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 70 kDa

species reactivity

human, mouse

application(s)

immunoprecipitation (IP): 1 μg/mL using lysate from 3T3/A31 cells
western blot: 1 μg/mL using lysate from 3T3/A31 cells

conjugate

unconjugated

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... MAP3K7(6885)
mouse ... Map3k7(26409)

General description

TAK1 is an orphan nuclear receptor that represses the transcription of Gata1 and also inhibits signal transduction via T3R, RAR and RXR pathways. This orphan receptor also regulates PPARα-mediated gene expression in liver cells . Anti-TAK1 antibody recognizes TAK1 (70 kDa). Additional unidentified bands may be detected.

Immunogen

synthetic peptide corresponding to amino acids 588-606 of human TAK1. The sequence is identical in mouse and Xenopus.

Application

Anti-TAK1 antibody is suitable for use in immunoblot (using whole-cell extracts from control MDA-MB-231 cells and MDA-MB-231 cells expressing shRNA to TAK1 ). The product can also be used for immunoprecipitation (1 μg using RIPA lysate (1 mg) from 3T3/HIR cells).

Physical form

Solution in 0.1 M Tris-glycine buffer, pH 7.4, containing 150 mM sodium chloride, 0.05% sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

nwg

Flash Point F

Not applicable

Flash Point C

Not applicable

T Hirose et al.
Biochemical and biophysical research communications, 211(1), 83-91 (1995-06-06)
Recently, we reported the cloning and characterization of the novel orphan receptor TAK1. In this study, we analyze the interaction of TAK1 with a variety of response elements (RE's) and demonstrate that TAK1 binds effectively to RE's composed of the...
Z H Yan et al.
The Journal of biological chemistry, 273(18), 10948-10957 (1998-06-06)
Recently, we reported the cloning of the nuclear orphan receptor TAK1. In this study, we characterized the sequence requirements for optimal TAK1 binding and analyzed the repression of the peroxisome proliferator-activated receptor alpha (PPARalpha) signaling pathway by TAK1. Site selection...
Osamu Tanabe et al.
Genes & development, 21(21), 2832-2844 (2007-11-03)
When the orphan nuclear receptors TR2 and TR4, the DNA-binding subunits of the DRED repressor complex, are forcibly expressed in erythroid cells of transgenic mice, embryos exhibit a transient mid-gestational anemia as a consequence of a reduction in the number...
Alfiya Safina et al.
Molecular cancer research : MCR, 9(8), 1042-1053 (2011-06-28)
Advanced-stage breast cancers frequently metastasize to the bones and cause bone destruction, but the underlying mechanism is not fully understood. This study presents evidence that TGF-β-activated protein kinase 1 (TAK1) signaling in tumor cells promotes bone destruction by metastatic breast...
Shin-Jen Lin et al.
Carcinogenesis, 35(6), 1399-1406 (2014-03-04)
Testicular nuclear receptor 4 (TR4), a member of the nuclear receptor superfamily, plays important roles in metabolism, fertility and aging. The linkage of TR4 functions in cancer progression, however, remains unclear. Using three different mouse models, we found TR4 could...

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