T8076

Sigma-Aldrich

Anti-TRB3 (N-terminal) antibody produced in rabbit

enhanced validation

affinity isolated antibody, buffered aqueous solution

Sinónimos:
Anti-SKIP3, Anti-Human tribbles 3 homolog, Anti-NIPK, Anti-TRIB3, Anti-SINK
MDL number:
NACRES:
NA.44
En este momento no podemos mostrarle ni los precios ni la disponibilidad

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 39 kDa

species reactivity

human

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

application(s)

western blot: 1-2 μg/mL using HEK-293 cells expressing human TRB3

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... TRIB3(57761)

Categorías relacionadas

General description

Tribbles homolog 3 (TRB3) gene is located on the 20p13 human chromosome. TRB3 and its related family members TRB1 and TRB2 show 45% sequence identity. It exhibits resemblance to ′tribbles′, a drosophila protein. TRB family members have a truncated kinase domain that lacks an adenosine 5′-triphosphate binding site (GXGXXG) and contain a variant catalytic core motif.

Immunogen

synthetic peptide corresponding to amino acids 11-29 located near the N-terminus region of human TRB3. The immunizing sequence has limited homology (~50% identity) to mouse and rat TRB3, and has no identity to TRB1 and TRB2 isoforms.

Application

Anti-TRB3 (N-terminal) antibody produced in rabbit has been used in immunoprecipitation and immunoblotting.

Biochem/physiol Actions

Tribbles homolog 3 (TRB3) like tribbles has also been identified as a potential pro-apoptotic protein, and is induced by a variety of cell stress conditions, including hypoxia and nutrient starvation. It was previously identified as a neuronal cell death-inducible protein kinase (NIPK) that is rapidly upregulated during neuronal apoptosis. TRB3 binds to activating transcription factor 4 (ATF4) and inhibits its transcriptional activity. TRB3 has been shown to interact with mitogen-activated protein kinases (MAPK) kinases to regulate activation of MAPK pathways.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point F

Not applicable

Flash Point C

Not applicable

TRB3: an oxidant stress-induced pseudokinase with a potential to negatively modulate MCP-1 cytokine in diabetic nephropathy
Kanwar YS
American Journal of Physiology: Renal Physiology, 299(5), F963-F964 (2010)
Role of TRIB3 in diabetic and overnutrition-induced atherosclerosis
Sowers JR
Diabetes, 61(2), 265-266 (2012)
Downregulation of TRB3 protects neurons against apoptosis induced by global cerebral ischemia and reperfusion injury in rats
Wei K, et al.
Neuroscience, 360, 118-127 (2017)
CHOP10/TRB3/Akt signaling regulates ER stress apoptosis in colon cancer cells treated with prostamide-J
Albassam H, et al.
American Journal of Physiology: Renal Physiology (2019)
Amino acid availability controls TRB3 transcription in liver through the GCN2eIF2alphaATF4 pathway
Carraro V, eta l.
PLoS ONE, 5(12), e15716-e15716 (2010)

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