T8154

Azul de tripano solution

0.4%, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): Blue cell viability dye
• Empirical Formula (Hill Notation): C₃₄H₂₄N₆Na₄O₁₄S₄
• CAS Number: 72-57-1
• Molecular Weight: 960.81
• Beilstein: 4360496
• MDL number: MFCD00003969
• PubChem Substance ID: 57654704
• NACRES: NA.75

Properties

• sterility: sterile-filtered
• Quality Level: 200
• form: liquid
• storage condition: dry at room temperature
• concentration: 0.4%
• technique(s): cell culture | mammalian: suitable; tissue processing: suitable
• application(s): cell analysis
• shipped in: ambient
• SMILES string: [Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
• InChI: 1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
• InChI key: GLNADSQYFUSGOU-UHFFFAOYSA-J

Description

General description

El azul de tripano es un colorante utilizado para distinguir entre células vivas y células muertas. Es un colorante vital que no es absorbido por las células viables sanas, pero tiñe las células con una membrana celular dañada. De esta forma, sólo pueden contarse las células muertas. Este método suele denominarse método de exclusión de colorante.

Trypan Blue (TB), an anionic hydrophilic azo dye, crosses only the cell membranes of dead cells, thereby staining dead tissues/cells blue. It is used in quantitative microscopy for a dye exclusion staining technique to differentiate between live and dead mammalian cells.

Application

Trypan Blue solution has been used:

• in cell viability assay to count viable and dead cells
• in rescue assay to count viable cells
• in trypan blue exclusion counting method to determine proliferation curve
• as the saline control and in the preparation of lipopolysaccharide (LPS) solution to confirm the success of LPS infusion
• in trypan blue dye exclusion test to determine gastric epithelial cells (GEC) viability
• to count GEC cells using a hemocytometer

Preparation Note

Se prepara en cloruro de sodio al 0,81% y en fosfato de potasio dibásico al 0,06%.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H350
• Precautionary Statements: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Carc. 1B
• Storage Class Code: 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves