T8158

Sigma-Aldrich

Transferrin human

powder, BioReagent, suitable for cell culture

Sinónimos:
Siderophilin, partially saturated
Número de CAS:
EC Number:
MDL number:
NACRES:
NA.75
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Quality Level

product line

BioReagent

assay

≥98%

form

powder

concentration

~25 mM

application(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested
hepatitis and HIV antibody, none detected

solubility

H2O: 50 mg/mL

shipped in

ambient

storage temp.

−20°C

Gene Information

human ... TF(7018)

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General description

Transferrin is a glycoprotein with a branched carbohydrate chain, having two sites for ferric ion binding in its polypeptide chain.

Application

Transferrin human has been used:
  • to incubate human proximal tubule cells (HKC) - 8 cells along with IgG for the examination of albumin specificity
  • as a supplement in serum-containing/devoid medium for human thyroid cell culture
  • as a target glycoprotein to develop an in-gel hydrazinolysis method

Biochem/physiol Actions

Transferrin mediates iron transportation from plasma to cells. The iron-bound transferrin are of three forms: monoferric A/B transferrin and diferric transferrin. The transferrin binds to specific cell surface receptors and are internalized by endocytosis. Once inside, the iron is released into the cells and the iron-free transferrin, also referred to as apotransferrin, is exocytosed outside the cell without being degraded. Transferrin is known to deliver iron to cells in a physiologically safe and effective form.

Other Notes

Partially iron-saturated

Preparation Note

Heat treated at 60 °C for ten hours.

Analysis Note

Purity by agarose electrophoresis.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point F

Not applicable

Flash Point C

Not applicable

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the carbohydrate content of human transferrin?

    We have found the information on transferrin in a reference book:The Plasma Proteins, F.W. Putnam, ed, volume 2, chapter 4, Table II.According to the table, Transferrin is 6 percent carbohydrate, citing G.A. Jamieson, J. Biol. Chem. 240(7), 2914-2920 (1965).The table also gives information on the percentage of each type of monosaccharide that is present.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Non-transferrin bound iron: a key role in iron overload and iron toxicity
Brissot P, et al.
Biochim. Biophys. Acta Gen. Subj., 1820(3), 403-410 (2012)
Detection of N-glycans on small amounts of glycoproteins in tissue samples and sodium dodecyl sulfate-polyacrylamide gels
Yoshimura T, et al.
Analytical Biochemistry, 423(2), 253-260 (2012)
Cells: Molecules and Mechanisms, 174-174 (2009)
Martijn F Hoes et al.
European journal of heart failure, 20(5), 910-919 (2018-02-28)
Iron deficiency is common in patients with heart failure and associated with a poor cardiac function and higher mortality. How iron deficiency impairs cardiac function on a cellular level in the human setting is unknown. This study aims to determine...
Eun-Min Kim et al.
PLoS neglected tropical diseases, 13(4), e0006843-e0006843 (2019-04-04)
Clonorchis sinensis is a group I bio-carcinogen responsible for cholangiocarcinoma (CHCA) in humans. However, the mechanism by which C. sinensis promotes carcinogenesis is unclear. Using the human cholangiocyte line H69, we investigated cell proliferation and gap junction protein expression after...
Artículos
How transferrin and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the <i>in vitro</i> chemistry and biochemistry of transferrin.
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