T9026

Sigma-Aldrich

Monoclonal Anti-α-Tubulin antibody produced in mouse

enhanced validation

clone DM1A, ascites fluid

Sinónimos:
Monoclonal Anti-α-Tubulin
NACRES:
NA.41
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Quality Level

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

DM1A, monoclonal

mol wt

antigen ~50 kDa

contains

15 mM sodium azide

species reactivity

bovine, rat, yeast, human, mouse, chicken, fungi, amphibian

enhanced validation

independent ( antibodies)
Learn more about Antibody Enhanced Validation

application(s)

indirect immunofluorescence: 1:500 using cultured chicken fibroblasts
western blot: 1:500 using human or chicken fibroblasts

isotype

IgG1

conjugate

unconjugated

Featured Industry

Research Pathology

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

¿Está buscando productos similares? Visit Guía de comparación de productos

Categorías relacionadas

General description

α-tubulin, also called tubulin α 4a (TUBA4A), is mapped to human chromosome 2q35. The gene codes for a member of the α-tubulin family, and contains 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α-subunit of tubulin has molecular weight of 50,000.
Monoclonal Anti-α-Tubulin (mouse IgG1 isotype) is derived from the DM1A hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Purified chick brain microtubules were used as immunogen. The isotype is determined by a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents, Product Number ISO2. The product is Protein A purified Monoclonal Anti-α-Tubulin antibody conjugated to fluorescein isothiocyanate, isomer I. It is purified by gel filtration and contains no detectable free FITC. Anti-α-Tubulin FITC antibody, Mouse monoclonal specifically recognizes an epitope in the carboxy terminal part of α-tubulin. It localizes α-tubulin in human, monkey, bovine, chicken, goat, murine, rat, gerbil, hamster, rat kangaroo, amphibia, sea urchin, trypanosome, yeast, fungi and tobacco.

Specificity

The antibody is specific for α-tubulin in immunoblotting assays and may be used for localization of α-tubulin in cultured cells or tissue sections. The antibody reacts best with chicken fibroblasts.

Immunogen

microtubules from chicken embryo brain.

Application

Monoclonal Anti-α-Tubulin antibody produced in mouse has been used in western blot analysis and protein expression analysis.
Monoclonal Anti-α-Tubulin antibody produced in mouse has been used in the detection of α-Tubulin:
  • in human osteosarcoma and in breast cancer cell lines by western blotting
  • in HeLa cells by immunofluorescence microscopy
  • by immunostaining
  • immunohistochemical detection in Xenopus embryos

Biochem/physiol Actions

Tubulin α 4a (TUBA4A) mutation is associated with amyotrophic lateral sclerosis (ALS).
α/β-Tubulin is the major building block of microtubules. These intracellular, hollow, cylindrical, filamentous structures are present in virtually all eukaryotic cells. Self-assembly of α/β-tubulin leads to polar microtubular structures built from linearly arranged strings of alternatively α- and β-tubulin pairs pointing in the same direction. Microtubules function as structural and mobile elements in mitosis, intracellular transport, ciliary flagellar motility and generation and maintenance of cell shape. α/β-Tubulin and γ-tubulin are members of the tubulin superfamily of proteins. α/β-Tubulin is a heterodimer which consists of one α-tubulin chain and one β-tubulin chain; each subunit has a molecular weight of 55 kDa and they share considerable homolog.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month.
For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point F

Not applicable

Flash Point C

Not applicable

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the immunogen for Monoclonal Anti-α-Tubulin antibody, clone DM1A?

    The immunogen for Monoclonal Anti-α-Tubulin antibody produced in mouse, clone DM1A is microtubules from chicken embryo brain.  This antibody recognizes an epitope located at the C-terminal end of the α-tubulin isoform (amino acids 426-430) as noted in the following references:Breitling, F., and Little, M., J. Molec. Biol., 189, 367-370 (1986).Wolff, A., et al., Biol. Cell, 63, 319-326 (1988).Serrano, L., et al., Anal. Biochem., 159, 253-259 (1986).

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

CHIP overexpression reduces mutant androgen receptor protein and ameliorates phenotypes of the spinal and bulbar muscular atrophy transgenic mouse model.
Adachi H
The Journal of Neuroscience, 27(19), 5115-5126 (2007)
TRAIL induces apoptosis in triple-negative breast cancer cells with a mesenchymal phenotype.
Rahman M, et al.
Breast Cancer Research and Treatment, 113(2), 217-230 (2009)
MID1 and MID2 are required for Xenopus neural tube closure through the regulation of microtubule organization.
Suzuki M, et al.
Development, dev-048769 (2010)
Analysis of gene function in somatic mammalian cells using small interfering RNAs.
Elbashir SM, et al.
Methods, 26(2), 199-213 (2002)
Telomere maintenance through spatial control of telomeric proteins.
Chen LY
Molecular and Cellular Biology, 27(16), 5898-5909 (2007)
Artículos
Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.
Más información
High titer lentiviral particles including beta-actin, alpha-tubulin and vimentin used for live cell analysis of cytoskeleton structure proteins.
Más información

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico

Redes sociales

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

Merck

Investigación. Desarrollo. Producción.

Somos un proveedor líder para la industria de Ciencias de la Vida con soluciones y servicios para investigación, desarrollo y producción biotecnológicos, y para desarrollo y producción de tratamientos farmacéuticos

© 2021 Merck KGaA, Darmstadt, Alemania y/o sus filiales. Todos los derechos reservados.

Queda estrictamente prohibida la reproducción sin permiso de cualquiera de los materiales de la página web.