T9069

Sigma-Aldrich

Topoisomerase I human

buffered aqueous glycerol solution, ≥2 units/μL

Comisión internacional de enzimas:
MDL number:
NACRES:
NA.54
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Quality Level

assay

≥2 units/μL

form

buffered aqueous glycerol solution

mol wt

100 kDa

concentration

≥2 units/μL

storage temp.

−70°C

Gene Information

human ... TOP1(7150)

General description

Topoisomerase I (TOP1) is mapped to human chromosome 20q12. TOP1 is a 91 kDa type IB enzyme and consists of N-terminal highly charged domain, a core, linker domain and a C-terminal catalytic domain with Tyr-723 active site residue.

Application

Topoisomerase I human has been used for Topoisomerase I assay for screening inhibitors.
Topoisomerase I has been used in a study to assess implications for the regulation of HIV-1 replication. Topoisomerase I has also been used in a study to investigate the tumor suppressor protein kinase Chk2 is a mediator of anoikis of intestinal epithelial cells.

Biochem/physiol Actions

Topoisomerase I human (TOP1) interacts with DNA by wrapping and takes a bi-lobed conformation. TOPI is essential for normal development and transcriptional regulation. Variants in the TOP1 gene is observed in patients with colorectal cancer.
Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of phosphodiester bonds in superhelical turns of closed-circular DNA. Enzyme activity is independent of right- and left-handed superhelices.

Unit Definition

One unit will relax 0.25 μg of supercoiled plasmid DNA in 30 minutes at pH 7.9 at 37 °C.

Physical form

Solution containing 20 mM sodium phosphate, pH 7.4, 300 mM NaCl, 50 μg/mL BSA, 50% glycerol, and between 25-100 mM imidazole (concentration will be lot dependent).

pictograms

Health hazard

signalword

Danger

hcodes

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point F

Not applicable

Flash Point C

Not applicable

Topoisomerase I in human disease pathogenesis and treatments
Li M and Liu Y
Genomics, Proteomics & Bioinformatics, 14(3), 166-171 (2016)
Binding and dissociation of human topoisomerase I with hairpin-loop RNAs: implications for the regulation of HIV-1 replication
Takahashi H, Sawa H, Hasegawa H,
Biochemical and Biophysical Research Communications, 297(3), 593-599 (2012)
M Lisby et al.
The Journal of biological chemistry, 276(23), 20220-20227 (2001-04-03)
All eukaryotic forms of DNA topoisomerase I contain an extensive and highly charged N-terminal domain. This domain contains several nuclear localization sequences and is essential for in vivo function of the enzyme. However, so far no direct function of the...
Novel insights into catalytic mechanism from a crystal structure of human topoisomerase I in complex with DNA
Redinbo MR, et al.
Biochemistry, 39(23), 6832-6840 (2000)
Molecular Networking Reveals Two Distinct Chemotypes in Pyrroloiminoquinone-Producing Tsitsikamma favus Sponges
Kalinski JCJ, et al.
Marine drugs, 17(1), 60-60 (2019)

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