Merck
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T9253

Sigma-Aldrich

Trypsin inhibitor from chicken egg white

Type II-O, Partially purified ovomucoid, containing ovoinhibitor

Synonym(s):
Ovomucoid
CAS Number:
Número de EC:
Número MDL:
NACRES:
NA.77

origen biológico

chicken egg white

Nivel de calidad

200

tpo

Type II-O

formulario

powder

mol peso

14 kDa

solubilidad

water: soluble 10 g/L

temp. de almacenamiento

2-8°C

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Descripción general

25% of ovomucoid comprises carbohydrate.
Ovomucoid or trypsin inhibitor is an abundant protein in most avian egg whites. The hen′s egg protein is composed of about 186 amino acid residues, and is highly glycosylated. Three tandem domains are each homologous with pancreatic trypsin inhibitor. It is highly immunogenic, and probably accounts for most cases of egg allergy.

Aplicación

Trypsin inhibitor from chicken egg white has been used:
  • to produce denatured, oxidized and deglycosylated ovomucoid and used to stimulate patient derived cell cultures for mapping T cell epitopes
  • to treat nuclei for flow cytometric analysis
  • in Holtfreter′s solution, used for the dissociation of planarians into cells

Envase

250, 500 mg in poly bottle
1, 5 g in poly bottle

Definición de unidad

One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.

Nota de análisis

One mg will inhibit 0.8-1.6 mg of tryspin with activity of approx. 10,000 BAEE units per mg protein. May inhibit ≤0.3 mg of chymotrypsin with activity of approx. 40 BTEE units per mg protein.

Otras notas

Ovomucoid, from chicken egg white, does not itself inhibit chymotrypsin. Chymotrypsin inhibition is a measure of ovoinhibitor contamination by method of Feeney, R. E., et al., J. Biol. Chem., 238, 1415 (1963).
View more information on Trypsin Inhibitor.

pictogramas

Health hazard

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Resp. Sens. 1 - Skin Sens. 1

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 3

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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Technical Information

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