Trypsin inhibitor from Phaseolus limensis (lima bean) has been used:
- in the inhibition of trypsin in human plasma
- in the termination of proteolytic digestion in rabbit skeletal muscles
- in the inhibition of salivary glands enzyme extract from Lygus lineolaris
- in the inhibition of proteolytic activity in Agave tequilana enzyme extract
Trypsin inhibitor has been used as an affinity ligand for isolation of elastase-type enzymes.
100, 500 mg in glass bottle
1 g in glass bottle
Acciones bioquímicas o fisiológicas
Trypsin inhibitor from lima beans belongs to Bowman-Birk family of protease inhibitors. It is 9 kDa in molecular weight and contains nine disulfide bridges and is highly thermostable. It has trypsin and chymotrypsin binding subdomains.
Monomer has an apparent molecular weight of approx. 9,000 Da but undergoes a concentration and pH-dependent dimerization.
Definición de unidad
One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.
Nota de preparación
Prepared by a modification of the method of Tauber, H., et al., J. Biol. Chem., 179, 1155 (1949) (modified).
Nota de análisis
One mg will inhibit ≥0.8 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.