Merck
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T9378

Sigma-Aldrich

Trypsin inhibitor from Phaseolus limensis (lima bean)

Type II-L, crude powder

Synonym(s):
Trypsin Inhibitor from lima beans
CAS Number:
Número de EC:
Número MDL:
NACRES:
NA.77

origen biológico

Phaseolus limensis (lima bean)

Nivel de calidad

200

tpo

Type II-L

formulario

crude powder

mol peso

9 kDa

solubilidad

0.067 M sodium phosphate buffer, pH 7.6: 1 mg/mL

temp. de almacenamiento

2-8°C

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Aplicación

Trypsin inhibitor from Phaseolus limensis (lima bean) has been used:
  • in the inhibition of trypsin in human plasma
  • in the termination of proteolytic digestion in rabbit skeletal muscles
  • in the inhibition of salivary glands enzyme extract from Lygus lineolaris
  • in the inhibition of proteolytic activity in Agave tequilana enzyme extract

Trypsin inhibitor has been used as an affinity ligand for isolation of elastase-type enzymes.

Envase

100, 500 mg in glass bottle
1 g in glass bottle

Acciones bioquímicas o fisiológicas

Trypsin inhibitor from lima beans belongs to Bowman-Birk family of protease inhibitors. It is 9 kDa in molecular weight and contains nine disulfide bridges and is highly thermostable. It has trypsin and chymotrypsin binding subdomains.

Propiedades físicas

Monomer has an apparent molecular weight of approx. 9,000 Da but undergoes a concentration and pH-dependent dimerization.

Definición de unidad

One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.

Nota de preparación

Prepared by a modification of the method of Tauber, H., et al., J. Biol. Chem., 179, 1155 (1949) (modified).

Nota de análisis

One mg will inhibit ≥0.8 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.

Otras notas

View more information on Trypsin Inhibitor.

pictogramas

Health hazard

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Resp. Sens. 1 - Skin Sens. 1

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 3

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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Product Information Sheet

Technical Information

SDS

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