T9574

Sigma-Aldrich

Anti-Tuberin (IA-22) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

MDL number:
NACRES:
NA.41
En este momento no podemos mostrarle ni los precios ni la disponibilidad

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 180-200 kDa

species reactivity

human, rat, mouse

application(s)

immunoprecipitation (IP): 5-10 μg using RIPA extract (0.5 mg) of human MCF-7 cells
western blot: 0.1-0.2 μg/mL using whole extracts of rat brain and mouse NIH3T3 cells

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... TSC1(7248)
mouse ... Tsc1(64930)
rat ... Tsc1(60445)

General description

Tuberin is encoded by the tuberous sclerosis complex subunit 2 (TSC2) gene which is located on the human chromosome at 16p13.3. Tuberin is found in mammalian cell lines and tissues. It co-localizes with hamartin in most tissues and cell types. Tuberin is present in cytosolic, microsomal, cytoskeletal, and vesicular fractions. This protein is localized in the nucleus of certain cells.

Specificity

Anti-Tuberin (IA-22) recognizes human, rat, and mouse tuberin.

Immunogen

synthetic peptide corresponding to amino acid residues 1463-1484 of human tuberin with N-terminal added cysteine, conjugated to KLH. The corresponding sequence differs by two amino acids in rat and mouse.

Application

Anti-Tuberin (IA-22) antibody produced in rabbit has been used in:
  • Chromatin immunoprecipitation (ChIP)
  • electrophoretic mobility shift assay (EMSA)
  • immunofluorescence staining
  • immunoprecipitation
  • immunoblotting

Packaging

200 μL in glass bottle

Biochem/physiol Actions

Tuberin and hamartin are involved in the regulation of cell cycle, cell growth and cell differentiation. It is also involved in cell adhesion and vesicular trafficking. Tuberin contains a guanidine triphosphatase (GTPase) activating protein homology (GAP) domain that is involved in the regulation of the small GTPase Ras homolog enriched in brain (Rheb) both in vitro and in vivo. Tuberin in combination with hamartin forms a functional cytoplasmic complex, that hampers growth by inhibiting phosphorylation of S6 kinase 1 (S6K1) and 4E binding protein 1 (4EBP1), probably through their upstream modulator mammalian target of rapamycin (mTOR). Mutations in the tuberous sclerosis complex subunit 2 (TSC2) gene are associated with tuberous sclerosis (TSC), an autosomal dominant hereditary disease that involves seizures, mental retardation, and benign tumors (hamartomas) in multiple organs including the kidney, brain, heart and skin. Tuberin and hamartin play a role in the phosphoinositide 3-kinase/ protein kinase B (Akt) signal transduction pathway.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificado de Análisis
Hamartin and tuberin expression in human tissues
Johnson M W, et al.
Modern Pathology, 14(3), 202-210 (2001)
Tuberin, the tuberous sclerosis complex 2 tumor suppressor gene product, regulates Rho activation, cell adhesion and migration
Astrinidis A, et al.
Oncogene, 21(55), 8470-8476 (2002)
Identification of the tuberous sclerosis complex-2 tumor suppressor gene product tuberin as a target of the phosphoinositide 3-kinase/akt pathway
Manning B D, et al.
Molecular Cell, 10(1), 151-162 (2002)
Loss of heterozygosity is detected at chromosomes 1p35-36 (NB), 3p25 (VHL), 16p13 (TSC2/PKD1), and 17p13 (TP53) in microdissected apocrine carcinomas of the breast.
Lininger R A, et al.
Modern Pathology, 12(12), 1083-1089 (1999)
Tuberous sclerosis complex gene products, Tuberin and Hamartin, control mTOR signaling by acting as a GTPase-activating protein complex toward Rheb
Tee A R, et al.
Current Biology, 13(15), 1259-1268 (2003)

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