Tuberin is encoded by the tuberous sclerosis complex subunit 2 (TSC2) gene which is located on the human chromosome at 16p13.3. Tuberin is found in mammalian cell lines and tissues. It co-localizes with hamartin in most tissues and cell types. Tuberin is present in cytosolic, microsomal, cytoskeletal, and vesicular fractions. This protein is localized in the nucleus of certain cells.
Anti-Tuberin (IA-22) recognizes human, rat, and mouse tuberin.
synthetic peptide corresponding to amino acid residues 1463-1484 of human tuberin with N-terminal added cysteine, conjugated to KLH. The corresponding sequence differs by two amino acids in rat and mouse.
Anti-Tuberin (IA-22) antibody produced in rabbit has been used in:
- Chromatin immunoprecipitation (ChIP)
- electrophoretic mobility shift assay (EMSA)
- immunofluorescence staining
200 μL in glass bottle
Tuberin and hamartin are involved in the regulation of cell cycle, cell growth and cell differentiation. It is also involved in cell adhesion and vesicular trafficking. Tuberin contains a guanidine triphosphatase (GTPase) activating protein homology (GAP) domain that is involved in the regulation of the small GTPase Ras homolog enriched in brain (Rheb) both in vitro and in vivo. Tuberin in combination with hamartin forms a functional cytoplasmic complex, that hampers growth by inhibiting phosphorylation of S6 kinase 1 (S6K1) and 4E binding protein 1 (4EBP1), probably through their upstream modulator mammalian target of rapamycin (mTOR). Mutations in the tuberous sclerosis complex subunit 2 (TSC2) gene are associated with tuberous sclerosis (TSC), an autosomal dominant hereditary disease that involves seizures, mental retardation, and benign tumors (hamartomas) in multiple organs including the kidney, brain, heart and skin. Tuberin and hamartin play a role in the phosphoinositide 3-kinase/ protein kinase B (Akt) signal transduction pathway.
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
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