U5379

Sigma-Aldrich

Anti-Ubiquitin antibody produced in rabbit

whole antiserum, lyophilized powder

MDL number:
NACRES:
NA.41
En este momento no podemos mostrarle ni los precios ni la disponibilidad

biological source

rabbit

Quality Level

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

mol wt

antigen 8.6 kDa

species reactivity

eukaryotes, prokaryotes

application(s)

immunohistochemistry (frozen sections): suitable
western blot: 1:100 using ubiquitin purified from bovine red blood cells

conjugate

unconjugated

UniProt accession no.

storage temp.

2-8°C

Gene Information

human ... UBB(7314), UBC(7316)
mouse ... Ubb(22187), Ubc(22190)
rat ... Ubb(192255), Ubc(50522)

General description

Ubiquitin is a highly conserved, 8.6kDa, eukaryotic cellular protein containing 76 amino acid residues. Ubiquitin is absent in prokaryotes.
Ubiquitin (UBB) gene is mapped to human chromosome 17p11.2.

Specificity

By immunoblotting, the antibody recognizes 1-3 protein bands using bovine red blood cell ubiquitin.

Immunogen

bovine red blood cell ubiquitin.

Application

Anti-Ubiquitin antibody produced in rabbit is used in:
  • immunostaining in neuropathology
  • immunohistochemistry
  • western blotting

Biochem/physiol Actions

Ubiquitin is involved in the selective degradation of many short-lived proteins by 26S proteasome. Proteins are targeted for degradation by covalent ligation to ubiquitin. This ubiquitin-mediated degradation of regulatory proteins plays crucial roles in the regulation of various processes, such as cell-cycle progression, signal transduction, transcriptional regulation, receptor down-regulation, stress response, protein trafficking and endocytosis. Ubiquitin protein also aids in immune response, metabolism, development, normal protein turnover, protein quality control by degrading misfolded and damaged proteins and apoptosis. Variation in the ubiquitin-mediated processes results in carcinogenesis.

Target description

Ubiquitin, present in both prokaryotes and eukaryotes, is a highly conserved 8.6 kDa protein. It is involved in the selective degradation of many short-lived proteins by the proteasome, an ATP-dependent protease. Proteins are targeted for degradation by covalent ligation to ubiquitin. This ubiquitin-mediated degradation of regulatory proteins plays important roles in the control of numerous processes, including cell-cycle progression, signal transduction, transcriptional regulation, receptor down-regulation, and endocytosis. It has also been implicated in immune response, metabolism, development, protein quality control, and apoptosis.

Physical form

Lyophilized from 0.01 M phosphate buffer, containing 1% lactose.

Reconstitution

Reconstitute the contents of the vial with 10 mL of Tris buffered saline. Since the antisera is preservative free, it is recommended that it be reconstituted in the presence of a preservative or aliquoted and frozen. Avoid repeated freeze/thaw cycles.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point F

Not applicable

Flash Point C

Not applicable

Mónika Lippai et al.
BioMed research international, 2014, 832704-832704 (2014-07-12)
The ubiquitin-proteasome system and autophagy were long viewed as independent, parallel degradation systems with no point of intersection. By now we know that these degradation pathways share certain substrates and regulatory molecules and show coordinated and compensatory function. Two ubiquitin-like...
Andrew Kertesz et al.
Brain : a journal of neurology, 128(Pt 9), 1996-2005 (2005-07-22)
This is a clinicopathologic study of a prospective, clinic-based cohort of patients with frontotemporal dementia (FTD)/Pick complex, who were followed to autopsy. A total of 60 patients with the clinical syndromes of the behavioural variant of FTD (FTD-bv) (n =...
Madoka Yoshida et al.
The EMBO journal, 25(9), 1934-1944 (2006-04-08)
The poly(A)-binding protein (PABP) is a unique translation initiation factor in that it binds to the mRNA 3' poly(A) tail and stimulates recruitment of the ribosome to the mRNA at the 5' end. PABP activity is tightly controlled by the...
Satoshi Sekiguchi et al.
The American journal of pathology, 169(5), 1722-1729 (2006-10-31)
Protein degradation is essential for oogenesis and embryogenesis. The ubiquitin-proteasome system regulates many cellular processes via the rapid degradation of specific proteins. Ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1) is exclusively expressed in neurons, testis, ovary, and placenta, each of which has...
Inez M J Meijer et al.
Cellular signalling, 23(2), 458-467 (2010-11-04)
Overexpression and poor downregulation of ErbB receptor tyrosine kinases are associated with enhanced signaling and tumorigenesis. Attenuation of EGF-receptor (EGFR) signaling is mediated by endocytosis and ubiquitination by the E3-ligase Cbl. En route to lysosomes, but before incorporation of the...

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