Merck
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U5379

Sigma-Aldrich

Anti-Ubiquitin antibody produced in rabbit

whole antiserum, lyophilized powder

Número MDL:
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

200

conjugado

unconjugated

forma del anticuerpo

whole antiserum

antibody product type

primary antibodies

clon

polyclonal

formulario

lyophilized powder

mol peso

antigen 8.6 kDa

species reactivity

eukaryotes, prokaryotes

technique(s)

immunohistochemistry (frozen sections): suitable
western blot: 1:100 using ubiquitin purified from bovine red blood cells

Nº de acceso UniProt

temp. de almacenamiento

2-8°C

Gene Information

Descripción general

Ubiquitin is a highly conserved, 8.6kDa, eukaryotic cellular protein containing 76 amino acid residues. Ubiquitin is absent in prokaryotes.
Ubiquitin (UBB) gene is mapped to human chromosome 17p11.2.

Especificidad

By immunoblotting, the antibody recognizes 1-3 protein bands using bovine red blood cell ubiquitin.

Inmunógeno

bovine red blood cell ubiquitin.

Aplicación

Anti-Ubiquitin antibody produced in rabbit is used in:
  • immunostaining in neuropathology
  • immunohistochemistry
  • western blotting

Acciones bioquímicas o fisiológicas

Ubiquitin is involved in the selective degradation of many short-lived proteins by 26S proteasome. Proteins are targeted for degradation by covalent ligation to ubiquitin. This ubiquitin-mediated degradation of regulatory proteins plays crucial roles in the regulation of various processes, such as cell-cycle progression, signal transduction, transcriptional regulation, receptor down-regulation, stress response, protein trafficking and endocytosis. Ubiquitin protein also aids in immune response, metabolism, development, normal protein turnover, protein quality control by degrading misfolded and damaged proteins and apoptosis. Variation in the ubiquitin-mediated processes results in carcinogenesis.

Descripción de destino

Ubiquitin, present in both prokaryotes and eukaryotes, is a highly conserved 8.6 kDa protein. It is involved in the selective degradation of many short-lived proteins by the proteasome, an ATP-dependent protease. Proteins are targeted for degradation by covalent ligation to ubiquitin. This ubiquitin-mediated degradation of regulatory proteins plays important roles in the control of numerous processes, including cell-cycle progression, signal transduction, transcriptional regulation, receptor down-regulation, and endocytosis. It has also been implicated in immune response, metabolism, development, protein quality control, and apoptosis.

Forma física

Lyophilized from 0.01 M phosphate buffer, containing 1% lactose.

Reconstitución

Reconstitute the contents of the vial with 10 mL of Tris buffered saline. Since the antisera is preservative free, it is recommended that it be reconstituted in the presence of a preservative or aliquoted and frozen. Avoid repeated freeze/thaw cycles.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

13 - Non Combustible Solids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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