U6260

Sigma-Aldrich

Ubiquitin-Specific Protease 10 human

recombinant, expressed in Sf9 cells, ≥56% (SDS-PAGE)

NACRES:
NA.54
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Quality Level

recombinant

expressed in Sf9 cells

assay

≥56% (SDS-PAGE)

form

buffered aqueous glycerol solution

mol wt

88 kDa

NCBI accession no.

shipped in

dry ice

storage temp.

−70°C

General description

Human USP10 (Ubiquitin-Specific Protease 10) or Ubiquitin C-terminal Hydrolase 10, amino acids 2-798 (end) with an N-terminal FLAG-tag, expressed in a baculovirus-infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

Ubiquitin-specific protease 10 (USP10) expression has been shown to be induced by vasopressin. In HEK293 cells, USP10 was shown to deubiquitylate sorting nexin 3.

Physical form

Solution in 40 mM Tris-HCl, 110 mM NaCl, 2.2 mM KCl, 80 ng/μl Flag peptide, 3 mM DTT, and 20% Glycerol at pH 8.0.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificado de Análisis
Vasopressin-inducible ubiquitin-specific protease 10 increases ENaC cell surface expression by deubiquitylating and stabilizing sorting nexin 3.
Boulkroun S.
American Journal of Physiology: Renal Physiology, 295, F889-F900 (2008)
C Soncini et al.
Oncogene, 20(29), 3869-3879 (2001-07-06)
Degradation of cellular proteins through ubiquitination is a fundamental strategy for regulating biological pathways. De-ubiquitination, i.e. the removal of ubiquitin from proteins and peptides to which ubiquitin is attached, is catalyzed by processing proteases known as de-ubiquitinating enzymes. We are...
Hortensia Faus et al.
Molecular and cellular endocrinology, 245(1-2), 138-146 (2005-12-22)
The role of the ubiquitin/proteasome system in degrading nuclear hormone receptors and regulating their transcriptional function has emerged in the last few years. We identified the ubiquitin-specific protease USP10 as part of DNA-bound androgen receptor (AR) complexes purified from nuclear...
Jian Yuan et al.
Cell, 140(3), 384-396 (2010-01-26)
Stability and localization of p53 is essential for its tumor suppressor function. Ubiquitination by the E3 ubiquitin ligase Mdm2 is the major regulatory mechanism of p53, which induces p53 nuclear export and degradation. However, it is unclear whether ubiquitinated cytoplasmic...

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