Merck
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V4014

Sigma-Aldrich

Anti-V5-Cy3 antibody, Mouse monoclonal

purified immunoglobulin, ~1 mg/mL, clone V5-10

Synonym(s):
Monoclonal Anti-V5
Número MDL:
NACRES:
NA.56

Nivel de calidad

200

origen biológico

mouse

conjugado

CY3 conjugate

forma del anticuerpo

purified immunoglobulin

antibody product type

primary antibodies

clon

V5-10, monoclonal

concentración

~1 mg/mL

technique(s)

immunocytochemistry: 2.0-4.0 μg/mL

enviado en

wet ice

temp. de almacenamiento

2-8°C

Descripción general

Monoclonal Anti-V5 (mouse IgG1 isotype) is derived from the V5 hybridoma, produced by fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acid residues GKPIPNPLLGLDST (95-108) of the P/V proteins of the Paramyxovirus SV5, conjugated to KLH. The conjugate is then extensively dialyzed to remove unbound Cy3 fluorophore. Monoclonal Anti-V5, Cy3 conjugate recognizes the V5 tag sequence on V5 tagged fusion proteins by immunocytochemistry.

Especificidad

Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins

Aplicación

Many recombinant proteins have been engineered to express a short sequence derived from the V5 molecule known as V5-tag. This tag facilitates the detection, isolation and purification of the proteins. Monoclonal antibodies reacting specifically with V5 and conjugated to Cy3 may be useful in various immunotechniques, and also to identify the expression of a V5 tagged fusion protein in situ by immunofluorescence.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Información legal

Cy3 is a trademark of Cytiva

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 2

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

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Certificate of Origin

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