V4014

Sigma-Aldrich

Monoclonal Anti-V5-Cy3 antibody produced in mouse

purified immunoglobulin, ~1 mg/mL, clone V5-10

Sinónimos:
Monoclonal Anti-V5
MDL number:
NACRES:
NA.56
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

V5-10, monoclonal

concentration

~1 mg/mL

application(s)

immunocytochemistry: 2.0-4.0 μg/mL

conjugate

CY3 conjugate

shipped in

wet ice

storage temp.

2-8°C

General description

Monoclonal Anti-V5 (mouse IgG1 isotype) is derived from the V5 hybridoma, produced by fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acid residues GKPIPNPLLGLDST (95-108) of the P/V proteins of the Paramyxovirus SV5, conjugated to KLH. The conjugate is then extensively dialyzed to remove unbound Cy3 fluorophore. Monoclonal Anti-V5, Cy3 conjugate recognizes the V5 tag sequence on V5 tagged fusion proteins by immunocytochemistry.

Specificity

Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins

Application

Many recombinant proteins have been engineered to express a short sequence derived from the V5 molecule known as V5-tag. This tag facilitates the detection, isolation and purification of the proteins. Monoclonal antibodies reacting specifically with V5 and conjugated to Cy3 may be useful in various immunotechniques, and also to identify the expression of a V5 tagged fusion protein in situ by immunofluorescence.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Legal Information

Cy3 is a trademark of Cytiva

Hazard Codes

Xn

Risk Statement

21/22

Safety Statement

36/37

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificado de Análisis
Dharmendra K Singh et al.
Molecular plant-microbe interactions : MPMI, 27(1), 7-17 (2013-09-21)
Protein kinase-driven phosphorylation constitutes the core of cellular signaling. Kinase components of signal transduction pathways are often targeted for inactivation by pathogens. The study of kinases and immune signal transduction in the model crop tomato (Solanum lycopersicum) would benefit from...
J A Southern et al.
The Journal of general virology, 72 ( Pt 7), 1551-1557 (1991-07-01)
Two canine isolates of simian virus 5 (SV5), termed CPI+ and CPI-, were examined for their ability to react with a bank of monoclonal antibodies (MAbs) that had been previously raised against a human isolate of SV5. CPI- virus was...
Junko Hasegawa-Minato et al.
Oncotarget, 9(3), 3483-3496 (2018-02-10)
c-Myc is a master regulator of various oncogenic functions in many types of human cancers. However, direct c-Myc-targeted therapy has not been successful in the clinic. Here, we explored a novel therapeutic target, which shows synthetic lethality in c-Myc-driven ovarian...
Suena Ji et al.
Nature communications, 9(1), 3508-3508 (2018-08-31)
Regulated secretion is an essential process where molecules destined for export are directed to membranous secretory granules, where they undergo packaging and maturation. Here, we identify a gene (pgant9) that influences the structure and shape of secretory granules within the...
S M Thomas et al.
Cell, 54(6), 891-902 (1988-09-09)
The "P" gene of the paramyxovirus SV5 encodes two known proteins, P (Mr approximately equal to 44,000) and V (Mr approximately equal to 24,000). The complete nucleotide sequence of the "P" gene has been obtained and is found to contain...

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