V8137

Anti-V5 antibody produced in rabbit

IgG fraction of antiserum

• Número MDL: MFCD03456076
• NACRES: NA.56

Properties

• origen biológico: rabbit
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: IgG fraction of antiserum
• antibody product type: primary antibodies
• clon: polyclonal
• technique(s): immunocytochemistry: 5 μg/mL using V5 tagged fusion protein in methanol-acetone fixed transiently transfected 293T cells; immunoprecipitation (IP): 5 μg/mL using V5 tagged fusion protein from extracts of transfected cells; western blot: 2.5 μg/mL using V5 tagged fusion protein expressed in whole extracts of transfected cells
• enviado en: dry ice
• temp. de almacenamiento: −20°C

Description

Descripción general

V5 is an amino coterminal protein consisting of a C-terminus cysteine-rich region that resembles a cysteine-rich metal binding domain. It is encoded by the "P" gene of the paramyxovirus SV5.
The rabbit Anti-V5 synthetic peptide antibody reacts with V5 tagged recombinant fusion proteins expressed in transfected mammalian cells and from in vitro translation.

Especificidad

Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins

Aplicación

Anti-V5 antibody produced in rabbit has been used in:

• immunocytochemistry
• immunoprecipitation
• western blot

Forma física

Solution in 0.01M phosphate buffered saline, pH 7.4 containing 15 mM sodium azide.

Almacenamiento y estabilidad

Store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• WGK: WGK 3
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)