V8137

Sigma-Aldrich

Anti-V5 antibody produced in rabbit

IgG fraction of antiserum

MDL number:
NACRES:
NA.56
En este momento no podemos mostrarle ni los precios ni la disponibilidad

biological source

rabbit

Quality Level

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

application(s)

immunocytochemistry: 5 μg/mL using V5 tagged fusion protein in methanol-acetone fixed transiently transfected 293T cells
immunoprecipitation (IP): 5 μg/mL using V5 tagged fusion protein from extracts of transfected cells
western blot: 2.5 μg/mL using V5 tagged fusion protein expressed in whole extracts of transfected cells

conjugate

unconjugated

shipped in

dry ice

storage temp.

−20°C

General description

V5 is an amino coterminal protein consisting of a C-terminus cysteine-rich region that resembles a cysteine-rich metal binding domain. It is encoded by the "P" gene of the paramyxovirus SV5.
The rabbit Anti-V5 synthetic peptide antibody reacts with V5 tagged recombinant fusion proteins expressed in transfected mammalian cells and from in vitro translation.

Specificity

Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins

Application

Anti-V5 antibody produced in rabbit has been used in:
  • immunocytochemistry
  • immunoprecipitation
  • western blot

Physical form

Solution in 0.01M phosphate buffered saline, pH 7.4 containing 15 mM sodium azide.

Storage and Stability

Store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificado de Análisis
Pyk2 is a Novel Tau Tyrosine Kinase that is Regulated by the Tyrosine Kinase Fyn
Chuanzhou Li and Jurgen G
Journal of Alzheimer's disease reports, 64(1), 205-221 (2018)
Mutations in MAPKBP1 Cause Juvenile or Late-Onset Cilia-Independent Nephronophthisis
Macia MS, et al.
American Journal of Human Genetics, 100(2), 323-333 (2017)
Fredrik Holmström et al.
Scientific reports, 6, 24991-24991 (2016-05-05)
The hepatitis C virus nonstructural (NS) 3/4A and NS5A proteins are major targets for the new direct-acting antiviral compounds. Both viral proteins have been suggested as modulators of the response to the host cell. We have shown that NS3/4A- and...
Bente B Johansson et al.
The Journal of biological chemistry, 286(40), 34593-34605 (2011-07-26)
CEL-maturity onset diabetes of the young (MODY), diabetes with pancreatic lipomatosis and exocrine dysfunction, is due to dominant frameshift mutations in the acinar cell carboxyl ester lipase gene (CEL). As Cel knock-out mice do not express the phenotype and the...
Ildar Gabaev et al.
Journal of virology, 88(24), 14326-14339 (2014-10-03)
The human cytomegalovirus (CMV) UL11 open reading frame (ORF) encodes a putative type I transmembrane glycoprotein which displays remarkable amino acid sequence variability among different CMV isolates, suggesting that it represents an important virulence factor. In a previous study, we...

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