Merck

V9264

Sigma-Aldrich

Anti-vinculina monoclonal antibody produced in mouse

clone hVIN-1, purified from hybridoma cell culture

Sinónimos:
Monoclonal Anti-Vinculin antibody produced in mouse
Número MDL:
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

purified immunoglobulin

antibody product type

primary antibodies

clon

hVIN-1, monoclonal

formulario

buffered aqueous solution

mol peso

antigen 116 kDa

species reactivity

frog, chicken, mouse, canine, human, bovine, rat, turkey

envase

antibody small pack of 25 μL

concentración

~1.0 mg/mL

technique(s)

immunocytochemistry: 5-10 μg/mL using HS-68 human fibroblast cell culture
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 0.05-0.1 μg/mL using HS-68 total cell extract

isotipo

IgG1

Nº de acceso UniProt

application(s)

research pathology

enviado en

dry ice

temp. de almacenamiento

−20°C

Gene Information

human ... VCL(7414)
mouse ... Vcl(22330)
rat ... Vcl(305679)

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Este artículo
SAB4200729F7053SAB4200080
conjugate

unconjugated

conjugate

-

conjugate

FITC conjugate

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture, purified immunoglobulin

clone

hVIN-1, monoclonal

clone

VIN-11-5, monoclonal

clone

hVIN-1, monoclonal

clone

V284, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

mol wt

antigen 116 kDa

mol wt

~120 kDa

mol wt

antigen 116 kDa

mol wt

antigen ~117 kDa

Descripción general

Anti-Vinculin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hVIN-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with vinculin purified from human uterus. Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell-extracellular matrix adherens-type junctions. In muscle, vinculin is localized in the fascia adherens of the intercalated disk (cardiac muscle), myotendinous junctions (skeletal muscle), neuromuscular junctions, and the membrane associated dense bodies of smooth muscle.

Especificidad

Marca de manera específica la vinculina en los contactos célula-célula y célula-sustrato. Reacciona fuertemente con la vinculina humana. Muestra reactividad cruzada con la metavinculina del músculo liso.

Inmunógeno

vinculina humana purificada del útero.

Aplicación

Anti-Vinculin antibody, Mouse monoclonal has been used in
  • immunoblotting
  • immunofluorescence staining
  • immunocytochemistry
  • immunohistochemistry and enzyme linked immunosorbent assay (ELISA).

Acciones bioquímicas o fisiológicas

Vinculin functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. In many cell types undergoing viral transformation, vinculin becomes redistributed to rosettes or podosomes.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Referencia del producto
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Tamaño del envase/cantidad

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