Merck
Todas las fotos(5)

V9264

Sigma-Aldrich

Anti-Vinculin antibody, Mouse monoclonal

clone hVIN-1, purified from hybridoma cell culture

Sinónimos:
Monoclonal Anti-Vinculin antibody produced in mouse
Número MDL:
NACRES:
NA.41

origen biológico

mouse

conjugado

unconjugated

forma del anticuerpo

purified immunoglobulin

antibody product type

primary antibodies

clon

hVIN-1, monoclonal

formulario

buffered aqueous solution

mol peso

antigen 116 kDa

species reactivity

bovine, canine, mouse, rat, turkey, human, chicken, frog

envase

antibody small pack of 25 μL

concentración

~1.0 mg/mL

technique(s)

immunocytochemistry: 5-10 μg/mL using HS-68 human fibroblast cell culture
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 0.05-0.1 μg/mL using HS-68 total cell extract

isotipo

IgG1

Nº de acceso UniProt

application(s)

research pathology

enviado en

dry ice

temp. de almacenamiento

−20°C

Gene Information

human ... VCL(7414)
mouse ... Vcl(22330)
rat ... Vcl(305679)

¿Está buscando productos similares? Visit Guía de comparación de productos

Descripción general

Anti-Vinculin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hVIN-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with vinculin purified from human uterus. Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell-extracellular matrix adherens-type junctions. In muscle, vinculin is localized in the fascia adherens of the intercalated disk (cardiac muscle), myotendinous junctions (skeletal muscle), neuromuscular junctions, and the membrane associated dense bodies of smooth muscle.

Especificidad

Specifically labels vinculin at cell-cell and cell-substrate contacts. Reacts strongly with human vinculin. Shows cross-reactivity with smooth muscle metavinculin.

Inmunógeno

purified human vinculin from uterus.

Aplicación

Anti-Vinculin antibody, Mouse monoclonal has been used in
  • immunoblotting
  • immunofluorescence staining
  • immunocytochemistry
  • immunohistochemistry and enzyme linked immunosorbent assay (ELISA).

Acciones bioquímicas o fisiológicas

Vinculin functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. In many cell types undergoing viral transformation, vinculin becomes redistributed to rosettes or podosomes.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificado de Análisis

Introduzca el número de lote para buscar un certificado de análisis (COA).

Certificado de origen

Introduzca el número de lote para buscar un Certificado de origen (COO).

Quotes and Ordering

Biomimetic calcium carbonate concentric microgrooves with tunable widths for promoting MC3T3-E1 cell functions
Wu X and Wang S
Advanced Helathcare Materials, 2(2), 326-333 (2013)
Shiqiong Hu et al.
Molecular biology of the cell, 22(17), 3120-3126 (2011-07-09)
Podosomes are dynamic, actin-containing adhesion structures that collectively self-organize as rings. In this study, we first show by observing osteoclasts plated on bead-seeded soft substrates that podosome assemblies, such as rings, are involved in tension forces. During the expansion of
Highly conserved testicular localization of claudin-11 in normal and impaired spermatogenesis
Stammler A, et al.
Testing, 11(8), e0160349-e0160349 (2016)
Functional importance of Dicer protein in the adaptive cellular response to hypoxia
Ho JJD, et al.
Test, 287(34), 29003-29020 (2012)
Masaki Mori et al.
Molecular biology of the cell, 20(13), 3115-3124 (2009-05-15)
Epithelial-mesenchymal transition (EMT) confers destabilization of cell-cell adhesion and cell motility required for morphogenesis or cancer metastasis. Here we report that zyxin, a focal adhesion-associated LIM protein, is essential for actin reorganization for cell migration in TGF-beta1-induced EMT in normal

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico