V9264

Anti-Vinculin antibody, Mouse monoclonal

clone hVIN-1, purified from hybridoma cell culture

• Sinónimos: Monoclonal Anti-Vinculin antibody produced in mouse
• Número MDL: MFCD00162905
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• conjugado: unconjugated
• forma del anticuerpo: purified immunoglobulin
• antibody product type: primary antibodies
• clon: hVIN-1, monoclonal
• formulario: buffered aqueous solution
• mol peso: antigen 116 kDa
• species reactivity: bovine, canine, mouse, rat, turkey, human, chicken, frog
• envase: antibody small pack of 25 μL
• concentración: ~1.0 mg/mL
• technique(s): immunocytochemistry: 5-10 μg/mL using HS-68 human fibroblast cell culture; immunohistochemistry: suitable; indirect ELISA: suitable; western blot: 0.05-0.1 μg/mL using HS-68 total cell extract
• isotipo: IgG1
• Nº de acceso UniProt: P18206
• application(s): research pathology
• enviado en: dry ice
• temp. de almacenamiento: −20°C
• Gene Information: human ... VCL(7414) ; mouse ... Vcl(22330) ; rat ... Vcl(305679)

Descripción

Descripción general

Anti-Vinculin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hVIN-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with vinculin purified from human uterus. Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell-extracellular matrix adherens-type junctions. In muscle, vinculin is localized in the fascia adherens of the intercalated disk (cardiac muscle), myotendinous junctions (skeletal muscle), neuromuscular junctions, and the membrane associated dense bodies of smooth muscle.

Especificidad

Specifically labels vinculin at cell-cell and cell-substrate contacts. Reacts strongly with human vinculin. Shows cross-reactivity with smooth muscle metavinculin.

Inmunógeno

purified human vinculin from uterus.

Aplicación

Anti-Vinculin antibody, Mouse monoclonal has been used in

• immunoblotting
• immunofluorescence staining
• immunocytochemistry
• immunohistochemistry and enzyme linked immunosorbent assay (ELISA).

Acciones bioquímicas o fisiológicas

Vinculin functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. In many cell types undergoing viral transformation, vinculin becomes redistributed to rosettes or podosomes.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)