Merck
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W0138

Sigma-Aldrich

Western Blocker Solution

for HRP detection systems

Synonym(s):
Anti-JNK-55, Anti-p195
NACRES:
NA.25

Nivel de calidad

200

caducidad

≥2 yr

temp. de almacenamiento

2-8°C

Descripción general

Western Blocker Solution is used to block both PVDF and nitrocellulose membranes after proteins have been transferred onto them through dot blotting or Western blotting; blocking is performed to reduce or eliminate nonspecific binding of primary or secondary antibodies.

Aplicación

Western Blocker Solution has been optimized for detection systems using horseradish peroxidase (HRP) conjugates; it is also compatible with alkaline phosphatase based systems.
Western Blocker Solution has been used for blocking of western blot membranes. It has also been used for dilution of antibodies in western blot.

Características y beneficios

  • Does not inhibit weak HRP signals, as many milk-based blockers do.
  • Can be used both as a blocking component and as a probing component.
  • Supplied ready-to-use; no preparative step is required.

Información legal

Western Blocker is a trademark of Sigma-Aldrich Co. LLC

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 2

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

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Certificate of Origin

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Quotes and Ordering

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