Williams′ Medium E

With sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

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Quality Level






cell culture | mammalian: suitable


endotoxin, tested


phenol red: no
glucose: 2.0 g/L (Dextro)
sodium pyruvate: 0.025 g/L
L-glutamine: no
NaHCO3: 2.2 g/L

shipped in


storage temp.


General description

Williams′ Medium E was developed by Williams and Gunn as a modification to Williams′ Medium D. The medium is formulated to support long-term cell culture of adult liver epithelial cells. It is a modified Minimum Essential Medium (MEM) with altered glucose and amino acid content.


Williams′ Medium E has been used in the culturing of human hepatocellular carcinoma (HCC) cells and hepatic cells.


Supplement with 0.292 g/L L-glutamine.

Other Notes

Phenol red has been shown to interfere with the growth of some cells at low or cloning densities. Use this version of William's E when working with stem cells or cells at low densities.


NONH for all modes of transport

WGK Germany


Flash Point F

Not applicable

Flash Point C

Not applicable

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Drug-induced skin sensitization is prevalent worldwide and can trigger life-threatening health conditions, such as Stevens Johnson Syndrome. However, existing in vitro skin models cannot adequately predict the skin sensitization effects of drugs administered into the systemic circulation because dermal inflammation...

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