XNAB2

Sigma-Aldrich

Extract-N-Amp Blood PCR Kit

sufficient for 100 extractions, sufficient for 100 amplifications

NACRES:
NA.55
En este momento no podemos mostrarle ni los precios ni la disponibilidad

usage

sufficient for 100 amplifications
sufficient for 100 extractions

Quality Level

color

colorless

shipped in

wet ice

storage temp.

−20°C

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General description

The Extract-N-Amp Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by PCR (Fig. 1). This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The kit also includes a PCR ReadyMix, specially formulated for amplification directly from the extract. This formulation uses an antibody based hot start, for specific amplification.

Application

Extract-N-Amp Blood PCR Kit has been used to extract DNA from genomes and dried blood spot samples. It has also been used for polymerase chain reaction (PCR).

Features and Benefits

  • Efficient 8 minute prep allows greater speed and throughput
  • No need for any type of purification, organic extraction, centrifugation or alcohol precipitation
  • Simple, 3 step procedure with no special equipment required
  • Hot start antibody included for highly specific PCR amplification of genomic DNA
  • Compatible with multiple format (single tube or 96-well plate)
  • Can be used with whole blood or blood cards
  • Extract stable at 4 °C for at least 6 months (Fig. 2)

Principle

Genomic DNA is extracted from 10 μl of whole blood by simply adding the Extraction Solution and incubating for 5 minutes at room temperature. The Neutralization Solution is added to the extract to counteract inhibitory substances prior to PCR. A portion of the DNA extract is then added to the specially formulated PCR ReadyMix.

Other Notes

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

pictograms

Corrosion

signalword

Danger

hcodes

RIDADR

UN 3316 9

Maria C Walsh et al.
PloS one, 6(11), e27177-e27177 (2011-12-02)
We assessed the effect of short-term feeding of genetically modified (GM: Bt MON810) maize on immune responses and growth in weanling pigs and determined the fate of the transgenic DNA and protein in-vivo. Pigs were fed a diet containing 38.9%...
Bo G Winkel et al.
BMC medical genetics, 12, 22-22 (2011-02-11)
The use of dried blood spots (DBS) samples in genomic workup has been limited by the relative low amounts of genomic DNA (gDNA) they contain. It remains to be proven that whole genome amplified DNA (wgaDNA) from stored DBS samples...
Roger G Rank et al.
Infection and immunity, 77(3), 1216-1221 (2009-01-14)
Over the last several years, four different phages of chlamydiae, in addition to a phage associated with Chlamydia psittaci isolated from an ornithosis infection in ducks over 25 years ago, have been described and characterized. While these phages and their...
Mads Vilhelm Hollegaard et al.
Molecular genetics and metabolism, 108(4), 225-231 (2013-02-21)
DNA methylation is the most common DNA modification and perhaps the best described epigenetic modification. It is believed to be important for genomic imprinting and gene regulation and has been associated with the development of diseases such as schizophrenia and...
Min Lu et al.
Blood, 120(15), 3098-3105 (2012-08-09)
Interferon (IFN-α) is effective therapy for polycythemia vera (PV) patients, but it is frequently interrupted because of adverse events. To permit the long-term use of IFN, we propose combining low doses of IFN with Nutlin-3, an antagonist of MDM2, which...
Artículos
The Extract-N-Amp Blood PCR Kits contain all the reagents needed to rapidly extract and amplify human genomic DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.
Más información
Extract-N-Amp is a versatile, combined DNA extraction and amplification kit intended to simplify the generation of PCR products from a range of sample types. This methodology can be used without modification to perform PCR on blood samples stored on FTA blood cards (Whatman) which removes the need for the laborious and costly washing procedures that the standard FTA protocol stipulates. This technique removes the variability between sample preparations and maximizes the number of PCR reactions that can be performed on individual FTA blood card samples.
Más información
The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.
Más información

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