XNAPR

Sigma-Aldrich

REDExtract-N-Amp Plant PCR Kit

sufficient for 1000 extractions, sufficient for 1000 amplifications

NACRES:
NA.55
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Quality Level

usage

sufficient for 1000 amplifications
sufficient for 1000 extractions

feature

hotstart

color

red

Featured Industry

Agriculture

storage temp.

−20°C

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General description

The REDExtract-N-Amp Plant PCR Kits contain all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. The REDExtract-N-Amp PCR ReadyMix contains a dye that acts as a tracking dye and allows for convenient direct loading of PCR reactions onto agarose gels for analysis.

Application

REDExtract-N-Amp Plant PCR Kit has been used to extract genomic DNA from leaves and mycelial lawns. It has also been used for polymerase chain reaction.

Features and Benefits

  • Single-step extraction of plant genomic DNA for PCR in less than 15 minutes
  • No freezing, mechanical disruption, organic extraction, column purification or precipitation required
  • Specially formulated PCR ReadyMix for use with extract
  • Hot Start antibody for highly specific PCR amplification of genomic DNA
  • REDExtract-N-Amp requires no loading buffers or tracking dyes required for gel analysis
  • Compatible with high-throughput requirements for genetic analysis of plants
  • Extract stable at 4 °C for at least 6 months (Fig. 3)

Components

Kits contain Dilution solution, Extraction solution, and REDExtract-N-Amp PCR Reaction Mix.

Other Notes

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

RIDADR

NONH for all modes of transport

Flash Point F

Not applicable

Flash Point C

Not applicable

Rowan S McKibbin et al.
Plant biotechnology journal, 4(4), 409-418 (2006-12-21)
Transgenic potato (Solanum tuberosum cv. Prairie) lines were produced over-expressing a sucrose non-fermenting-1-related protein kinase-1 gene (SnRK1) under the control of a patatin (tuber-specific) promoter. SnRK1 activity in the tubers of three independent transgenic lines was increased by 55%-167% compared...
Neil D Havis et al.
FEMS microbiology letters, 256(2), 217-223 (2006-02-28)
Ramularia collo-cygni is a barley pathogen of increasing importance in Northern and Central Europe, New Zealand and South America. Accurate visual and microscopic identification of the pathogen from diseased tissue is difficult. A nested PCR-based diagnostic test has been developed...
Anna Stöger et al.
Journal of microbiological methods, 58(2), 281-284 (2004-07-06)
Angular leaf spot is a bacterial disease caused by Xanthomonas fragariae. It has become a serious disease in the USA and Europe in recent years. Several detection procedures are described for this plant pathogen. However, they are either too time-consuming...
José F Cobo-Díaz et al.
Systematic and applied microbiology, 37(3), 177-185 (2013-11-26)
Genista versicolor is an endemic legume from Sierra Nevada National Park which constitutes one of the UNESCO-recognized Biosphere Reserves. In the present study, a collection of strains nodulating this legume was analysed in characteristic soils of this ecosystem. Most strains...
Martha E Trujillo et al.
The ISME journal, 4(10), 1265-1281 (2010-05-07)
Our current knowledge of plant-microbe interactions indicate that populations inhabiting a host plant are not restricted to a single microbial species but comprise several genera and species. No one knows if communities inside plants interact, and it has been speculated...
Artículos
The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that are available and help you find the best kit suited for your needs.
Más información
The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.
Más información
Protocolos
The REDExtract-N-Amp Plant PCR Kits contain all the reagents needed to rapidly extract and amplify genomic DNA from plant leaves.
Más información

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