XNASS

Sigma-Aldrich

REDExtract-N-Amp Seed PCR Kit

sufficient for 10 extractions, sufficient for 10 amplifications

NACRES:
NA.55
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Quality Level

usage

sufficient for 10 amplifications
sufficient for 10 extractions

shipped in

dry ice

storage temp.

−20°C

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General description

The REDExtract-N-Amp Seed PCR Kits provide all the reagents necessary to rapidly extract DNA from a wide variety of plant seeds and amplify targets of interest by PCR. A novel extraction method eliminates the need for long enzymatic digestions. The kit also includes a specially formulated hot start PCR reaction mix for amplification directly from the extract. The REDExtract-N-Amp PCR ReadyMix contains an inert dye that acts as a tracking dye and allows for convenient loading of PCR reactions onto agarose gels for analysis.

The kit comes with validated protocols to extract and amplify genomic DNA from corn, wheat, sunflower, cotton, soybean, sorghum, canola, and Arabidopsis seeds. Additional protocols are available. In a typical procedure, genomic DNA is extracted from a sample that has been ground and incubated in the Seed Preparation Solution and Extraction Solution for 10 minutes at room temperature. The sample is heated to 95 °C for 3 minutes and then mixed with a third solution to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and the REDExtract-N-Amp PCR ReadyMix, included in the kit.

Application

REDExtract-N-Amp seed PCR Kit has been used for DNA extraction.

Features and Benefits

  • Perfect for quick genotyping results
  • Rapid extraction of genomic DNA for PCR in 15 minutes
  • No long enzymatic digestions
  • Hot Start antibody for highly specific PCR amplification of genomic DNA

Principle

The kit comes with validated protocols to extract and amplify genomic DNA from corn, wheat, sunflower, cotton, soybean, sorghum, canola, and Arabidopsis seeds. Additional protocols are available. In a typical procedure, genomic DNA is extracted from a sample that has been ground and incubated in the seed preparation solution and extraction solution for 10 minutes at room temperature. The sample is heated to 95 °C for 3 minutes and then mixed with a third solution to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and the Extract-N-Amp PCR Reaction Mix (included in the kit) and PCR is initiated.

Other Notes

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Legal Information

Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries. SYBR Green nucleic acid stain is licensed from Molecular Probes, Inc. under US Patent No. 5,436,134 for use in Real-Time PCR by end-users for research and development only. SYBR is a registered trademark of Molecular Probes, Inc.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

RIDADR

NONH for all modes of transport

Certificado de Análisis
Marko Stojkovic et al.
Journal of separation science, 35(24), 3509-3513 (2012-12-12)
The use of CE with contactless conductivity detection for the determination of PCR products is demonstrated for the first time. The separation of specific length PCR products according to their size could be achieved using 5% PVP as a sieving...
Angela Quarta et al.
FEMS microbiology letters, 259(1), 7-13 (2006-05-11)
The ability to rapidly distinguish trichothecene chemotypes in a given species/population of the genus Fusarium is important due to significant differences in the toxicity of these secondary metabolites. A multiplex PCR assay, based on primer pairs derived from the Tri3...
Artículos
The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.
Más información
Protocolos
The REDExtract-N-Amp Seed PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from seeds (soybean, corn, wheat, etc.).
Más información

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