Y2377

2x YT medium

Powder microbial growth medium

• Synonym(s): 2x Yeast Extract Tryptone medium, 2xTY medium, 2xYT Medium, 2xYT media
• NACRES: NA.85

Properties

• grade: for molecular biology
• sterility: non-sterile
• form: powder
• pH: 6.8-7.2(3.1% solution)
• application(s): food and beverages
• storage temp.: room temp
• suitability: nonselective for Escherichia coli; nonselective for coliforms

Description

General description

2xYT (2x Yeast Extract Tryptone medium) is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Application

2x YT medium has been used for culturing E. coli BL21 for cloning , E. coli SS320 cells for scaffold synthesis , and NEB 5-alpha competent E. coli cells for protospacer adjacent motif (PAM) binding assays. It has also been used in the culturing of Agrobacterium tumefaciens and E. coli DH10B.

Features and Benefits

2xYT powder provides:

• Standard formulation
• An inexpensive alternative to liquid

Components

16 g/L Tryptone
10 g/L Yeast Extract
5 g/L NaCl

Preparation Note

1. Stir to suspend 31 g of 2xYT Microbial Medium Powder in 1 L of water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121 °C.

Reconstitution

Add 31 g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves