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ZMS1041

Sigma-Aldrich

Anti-MDM2 Antibody, clone IF2 ZooMAb® Mouse Monoclonal

newenhanced validation

recombinant, expressed in HEK 293 cells

Quality Level

biological source

mouse

recombinant

expressed in HEK 293 cells

antibody form

purified antibody

antibody product type

primary antibodies

clone

IF2, recombinant monoclonal

description

recombinant, expressed in HEK 293 cells

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 55.23 kDa
observed mol wt ~75 kDa

purified by

using protein G

species reactivity

human

packaging

antibody small pack of 25 μL

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

application(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG

epitope sequence

N-terminal

conjugate

unconjugated

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

General description

ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone IF2 is a ZooMAb® Mouse recombinant monoclonal antibody that detects MDM2.

Immunogen

Recombinant human MDM2.

Application

Anti-MDM2, clone IF2 ZooMAb®, Cat. No. ZMS1041, is a recombinant Mouse monoclonal antibody that detects MDM2 and is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Quality Control Testing

Evaluated by Western Blotting in lysate from Nutlin 3a treated U2OS cells.

Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected MDM2 in lysate from Nutlin 3a treated U2OS cells, but not in lysate from untreated cells.

Tested applications

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected MDM2 in U2OS cells treated with Nutlin-3a.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MDM2 in human testis cancer and human stomach cancer tissue sections.

Flow Cytometry Analysis: 1 μg from a representative lot detected MDM2 in one million U2OS cells treated with Nutlin-3a.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

E3 ubiquitin-protein ligase Mdm2 (UniProt: Q00987; also known as EC:2.3.2.27, Double minute 2 protein, Hdm2, Oncoprotein Mdm2, RING-type E3 ubiquitin transferase, Mdm2, p53-binding protein Mdm2) is encoded by the MDM2 gene (Gene ID: 4193) in human. Mdm2 is a ubiquitously expressed, p53-inducible phosphoprotein protein that is predominantly expressed in the nucleoplasm. It binds to the N-terminus of the p53 and negatively regulates its activity. as an E3 ligase to ubiquitinate p53 and force its export from the nucleus to the cytoplasm where it is degraded in the proteasome. Over-expression of MDM2 is reported in several cancers, including breast cancer and soft tissue carcinomas. In breast cancer its over-expression is also correlated with lack of p21 expression. MDM2 overexpression is also associated with chemotherapeutic resistance in human malignancies. The mechanisms of chemotherapeutic resistance by MDM2 overexpression includes both the p53-MDM2 loop-dependent and p53-MDM2 loop-independent pathways. In normal cells, its over-expression causes G1 phase arrest. Hence, in normal cells it may have a protective role in preventing untimely cell cycle progression. MDM2 can underdo phosphorylation on serine 166 by SGK1 and at multiple sites near the RING domain (aa 438-479) by ATM kinase upon DNA damage. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Hou, H., et al. (2019). Cancer Cell Int. 19; Article 216).

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

13 - Non Combustible Solids

WGK Germany

WGK 1

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificado de Análisis

Certificado de origen

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