Todas las fotos(12)



Anti-GST Antibody, clone 5B8 , ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

Glutathione S-transferase

origen biológico

rabbit (recombinant)

Nivel de calidad


expressed in HEK 293 cells



forma del anticuerpo

purified antibody

antibody product type

primary antibodies


5B8, recombinant monoclonal

Línea del producto

ZooMAb® learn more



mol peso

calculated mol wt 22.87 kDa
observed mol wt ~26 kDa

species reactivity



antibody small pack of 25 μL

características de los productos alternativos más sostenibles

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

validación mejorada

recombinant expression
Learn more about Antibody Enhanced Validation


ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
western blot: suitable



categoría alternativa más sostenible

enviado en


temp. de almacenamiento


Gene Information

human ... GSTA1(2938)

Comparar elementos similares

Ver comparación completa

Show Differences

1 of 4

Este artículo

expressed in HEK 293 cells


expressed in HEK 293 cells


expressed in HEK 293 cells


expressed in HEK 293 cells









antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody


5B8, recombinant monoclonal


2J11, recombinant monoclonal


1F4, recombinant monoclonal


1B11, recombinant monoclonal

product line

ZooMAb® learn more

product line

ZooMAb® learn more

product line

ZooMAb® learn more

product line

ZooMAb® learn more

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.


Clone 5B8 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Glutathione S Transferase.


Full-length recombinant E. Coli Glutathione S-transferase.


Anti-GST, clone 5B8 ZooMAb, Cat. No. ZRB1223, is a recombinant Rabbit monoclonal antibody that specifically detects Glutathione S Transferase and has been tested for use in Dot Blot, ELISA, Immunocytochemistry and Western Blotting.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GST in HeLa cells transfeced with GST expression plasmid.

ELISA Analysis: 0.5 μg/mL from a representative lot detected GST on plates coated with in GST, GST-TBP (Tata-Bindidng protein), and GST-beta-catenin.

Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected various concentrations of GST-Tagged recombinant protein (portion of CD25 from amino acids 22-140), but not the His-Tagged recombinant protein.

Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.

Descripción de destino

Glutathione S-transferase (also known as GST) is encoded by the Gst gene in E. coli. Glutathione S transferases are a group of enzymes that are responsible for the detoxification of reactive chemical species through conjugation to reduced glutathione. They are ubiquitously distributed in nature, being found in organisms from microbes to higher mammals. They exist as membrane-bound as well as cytosolic enzymes with distinct catalytic and noncatalytic binding properties. They catalyze the conjugation of reduced glutathione (GSH) with compounds that contain an electrophilic center through the formation of a thioether bond between the sulfur atom of GSH and the substrate. The fundamental basis of their catalytic activity has been attributed to their capacity to lower the pKa of the sulfhydryl group of GSH from 9.0 in aqueous solution to about 6.5 when GSH is bound in the active site. In the case of certain substrates, GST can catalyze both the forward and reverse reactions, which can lead to increased toxicity rather than detoxication. GST has also been used as a tag for recombinant proteins where it can be fused with either the N- or the C-terminal of protein. Proteins produced using the GST fusion system have been used in numerous biological applications. It allows proteins to become more soluble. Based on their biochemical, immunological, and structural properties, the soluble human GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Nebert, DW., and Vasiliou, V. (2004). Hum. Genomics. 1(6); 460-464).

Forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.


30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

11 - Combustible Solids



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Las referencias de los producto se combinan con los tamaños de envase y las cantidades cuando se muestran en la página web, por ejemplo: T1503-25G. Asegúrese de introducir el número de producto ÚNICAMENTE en el campo del Número de producto (Ejemplo T1503).


Referencia del producto
Tamaño del envase/cantidad

Otros ejemplos





introducir como1.000309185)

¿Tiene algún problema? No dude en ponerse en contacto con nosotros Servicio técnico para asistencia

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Productos Aldrich

  • Si encuentra un número de lote como TO09019TO, introduzca el número de lote 09019TO sin las dos primeras letras.

  • Si encuentra un número de lote con un código de entrada como 05427ES-021, introduzca el número 05427ES sin el código de entrada -021.

  • Si encuentra un número de lote con un código de entrada como STBB0728K9, introduzca el código de lote STBB0728 sin el código de entrada K9.

¿No ha encontrado lo que estaba buscando?

En algunos casos, puede que un COA no esté disponible en la página web. Si su búsqueda no encontró el COA puede solicitarlo.

Solicitar COA

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico