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ZRB1489

Sigma-Aldrich

Anti-BMP-2 Antibody, clone 4O5 ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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Sinónimos:
BMP-2A, Bone morphogenetic protein 2, Bone morphogenetic protein 2A

biological source

rabbit (recombinant)

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

4O5, monoclonal
recombinant monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 44.7 kDa
observed mol wt 38 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable

isotype

IgG

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... BMP2(650)

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ZRB1627ZRB1375ZRB1155
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

species reactivity

human

species reactivity

human

species reactivity

rat, human, mouse

species reactivity

mouse, monkey, human

Gene Information

human ... BMP2(650)

Gene Information

-

Gene Information

human ... BCL2L11(10018)

Gene Information

-

biological source

rabbit (recombinant)

biological source

rabbit

biological source

rabbit (recombinant)

biological source

rabbit

clone

4O5, monoclonal, recombinant monoclonal

clone

recombinant monoclonal

clone

2O18, monoclonal, recombinant monoclonal

clone

recombinant monoclonal

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General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 4O5 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Bone morphogenetic protein 2 (BMP-2). It targets an epitope within the C-terminal half.

Immunogen

GST/His-tagged recombinant fragment corresponding to 101 amino acids from the C-terminal region of human BMP-2.

Application

Affinity Binding Assay: A representative lot of this antibody bound human recombinant BMP-2 with a KD of 1.8 x 10-8 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected BMP-2 in one million HepG2 cells.

Flow Cytometry Analysis: 0.1 μg from a representative lot detected BMP-2 in HepG2 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-BMP-2, clone 4O5 ZooMAb, Cat. No. ZRB1489, is a recombinant Rabbit monoclonal antibody that specifically targets BMP-2 and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, and Western Blotting.

Target description

Bone morphogenetic protein 2 (UniProt: P12643; also known as BMP-2, Bone morphogenetic protein 2A, BMP-2A) is encoded by the BMP2 (also known as BMP2A) gene (Gene ID: 650) in human. BMP-2 is a member of the transforming growth factor-beta (TGF-beta) superfamily. It is synthesized as a 60-kDa precursor with a signal peptide (aa 1-23) and a propeptide (aa 24-282) which is processed in the secretory pathway to a small 18-kDa monomer. Two monomers associate to form the active homodimer that binds to its receptor. BMP-2 is shown to be abundant in lung, spleen, and colon. Lower but significant levels are also found in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary, and small intestine. BMP-2 induces cartilage and bone formation and stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP-2 activation of EIF2AK3 stimulates phosphorylation of EIF2A that leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. It is reported to increase the colony formation of normal human bone marrow stromal preosteoblasts and induce their differentiation into cells with mature osteoblastic phenotype. BMP-2 is also known to activate osteoclast through receptor activator of nuclear kappa-B ligand (RANKL). This ZooMAb®recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1


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