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ZRB1733

Sigma-Aldrich

Anti-IDH1 Antibody, clone 1M9 ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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Sinónimos:
Isocitrate dehydrogenase [NADP] cytoplasmic;EC:1.1.1.42;IDH;Cytosolic NADP-isocitrate dehydrogenase;IDP;NADP(+)-specific ICDH;Oxalosuccinate decarboxylase

origen biológico

rabbit

Nivel de calidad

recombinante

expressed in HEK 293 cells

conjugado

unconjugated

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

1M9, recombinant monoclonal

descripción

recombinant, expressed in HEK 293 cells

Línea del producto

ZooMAb® learn more

formulario

lyophilized

mol peso

calculated mol wt 46.66 kDa
observed mol wt ~53 kDa

purificado por

using Protein A

reactividad de especies

mouse, rat

reactividad de especies (predicha por homología)

human, monkey

envase

antibody small pack of 25 μL

características de los productos alternativos más sostenibles

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

validación mejorada

recombinant expression
Learn more about Antibody Enhanced Validation

técnicas

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotipo

IgG

secuencia del epítopo

C-terminal half

Nº de acceso Protein ID

Nº de acceso UniProt

categoría alternativa más sostenible

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... IDH1(3417)

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Especificidad

Clone 1M9 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Isocitrate dehydrogenase [NADP] cytoplasmic (IDH1). It targets an epitope within 14 amino acids from the C-terminal half.

Inmunógeno

KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal half of human Isocitrate dehydrogenase [NADP] cytoplasmic (IDH1).

Aplicación

Quality Control Testing

Evaluated by Western Blotting in Mouse Kidney tissue lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected IDH1 in Mouse Kidney tissue lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected IDH1 in C2C12 and NIH3T3 cell lysates.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected IDH1 in NIH 3T3 cells.

Affinity Binding Assay: A representative lot of this antibody bound IDH1 with a KD of 5.9 x 10-10 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected IDH1 in mouse testis tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Descripción de destino

Isocitrate dehydrogenase [NADP] cytoplasmic (UniProt: EC:1.1.1.42, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase) is encoded by the IDH1 (also known as PICD) gene (Gene ID: 3417) in human. A total of five isocitrate dehydrogenases have been described: three of these are NAD(+)-dependent that localize to the mitochondrial matrix, and two are NADP(+)-dependent enzymes. One of the NADP(+) enzyme is mitochondrial and the other is predominantly localized in the cytoplasm. IDH1 is a homodimeric enzyme that is localized in cytoplasm and peroxisomes and catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. It has two NADP binding sites (aa 75-77 and 310-315) and plays a significant role in cytoplasmic NADPH production. Acetylation at Lys-374 is reported to significantly reduce its catalytic activity. Mutations in IDH1 gene, particularly those affecting Arg 132, have been linked to development of high-grade gliomas. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Reitman, ZJ., et al. (2011). Proc. Natl. Acad. Sci. USA. 108(8); 3270-3275; Dang, L., et al. (2009). Nature. 462(7274); 739-744).

Forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitución

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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