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ZRB2383

Sigma-Aldrich

Anti-GFAP Antibody, clone 2E8, ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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Sinónimos:
GFAP

origen biológico

rabbit (recombinant)

Nivel de calidad

recombinante

expressed in HEK 293 cells

conjugado

unconjugated

forma del anticuerpo

purified antibody

antibody product type

primary antibodies

clon

2E8, monoclonal
recombinant monoclonal

descripción

2E8 Clone

Línea del producto

ZooMAb® learn more

formulario

lyophilized

mol peso

calculated mol wt 49.88 kDa
observed mol wt ~51 kDa

species reactivity

human, rat, mouse

envase

antibody small pack of 25 μL

características de los productos alternativos más sostenibles

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

validación mejorada

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

immunocytochemistry: suitable using 1:100
immunofluorescence: suitable using 1:100
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using 1:100
western blot: suitable using 1:1,000

isotipo

IgG

Nº de acceso UniProt

categoría alternativa más sostenible

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

target post-translational modification

unmodified

Gene Information

human ... GFAP(2670)

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Este artículo
ZRB1648ZRB1013ZRB7356
recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

clone

2E8, monoclonal, recombinant monoclonal

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5B11, monoclonal, recombinant monoclonal

clone

2F8, recombinant monoclonal

clone

1B21, monoclonal, recombinant monoclonal

product line

ZooMAb® learn more

product line

ZooMAb® learn more

product line

ZooMAb® learn more

product line

ZooMAb® learn more

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Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Especificidad

Clone 2E8 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Glial fibrilary acidic protein (GFAP). It targets an epitope with in 11 amino acids from the C-terminal region.

Inmunógeno

KLH-conjugated linear peptide corresponding to 11 amino acids from the C-terminal region of human Glial fibrilary acidic protein (GFAP).

Aplicación

Anti-GFAP, clone 2E8, ZooMAb, Cat. No. ZRB2383, is a recombinant Rabbit Monoclonal Antibody that specifically targets Glial fibrillary acidic protein (GFAP) and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunfluorescence, and Western Blotting.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected GFAP in human brain and rat brain tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GFAP in C6 glioblastoma cells and primary cultures of rat glial precursor cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GFAP in rat brain and human brain tissue sections.

Descripción de destino

Glial fibrillary acidic protein (UniProt: P14136; also known as GFAP) is encoded by the GFAP gene (Gene ID: 2670) in human. GFAP is a class-III intermediate filament that serves as a cell-specific marker that can distinguish astrocytes from other glial cells. GFAP is not detected outside the central nervous system. It is expressed in cells that lack fibronectin. GFAP is a good marker of astroglial injury where its levels are consistently upregulated. GFAP levels are also shown to be upregulated with age in rodents and humans. Hence, it can serve as a surrogate marker of aging in the brain. GFAP has a relatively slow metabolic turnover rate, which is consistent with its structural role. GFAP is composed of three distinct regions: an N-terminal head region, a central ROD region, and a C-terminal tail region. The ROD regions are highly conserved among intermediate filament proteins. However, the size and amino acid sequences of the head and tail regions can vary widely. Three different isoforms of GFAP have been described that are produced by alternative splicing. Mutations in GFAP gene are known to cause Alexander disease that is characterized by progressive failure of central myelination, usually leading to death within the first decade. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitución

30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Xin Yi Ng et al.
NPJ Parkinson's disease, 9(1), 26-26 (2023-02-16)
Parkinson's disease (PD) is a neurodegenerative disorder characterized by defective dopaminergic (DAergic) input to the striatum. Mutations in two genes encoding synaptically enriched clathrin-uncoating factors, synaptojanin 1 (SJ1) and auxilin, have been implicated in atypical Parkinsonism. SJ1 knock-in (SJ1-KIRQ) mice

Contenido relacionado

Choose sustainable options for your research with greener alternative ZooMAb® recombinant antibodies. These BSA-free, carrier-free antibodies are shipped in low-waste packaging and offer maximum stability without compromising performance.

Elija opciones sostenibles para su investigación con los anticuerpos recombinantes ZooMAb® alternativos más ecológicos. Estos anticuerpos sin BSA ni portadores se envían en envases que generan pocos residuos y ofrecen la máxima estabilidad sin comprometer el rendimiento.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

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